| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UCK2 |
| Uniprot No | Q9BZX2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-261aa |
| 氨基酸序列 | MAGDSEQTLQNHQQPNGGEPFLIGVSGGTASGKSSVCAKIVQLLGQNEVD YRQKQVVILSQDSFYRVLTSEQKAKALKGQFNFDHPDAFDNELILKTLKE ITEGKTVQIPVYDFVSHSRKEETVTVYPADVVLFEGILAFYSQEVRDLFQ MKLFVDTDADTRLSRRVLRDISERGRDLEQILSQYITFVKPAFEEFCLPT KKYADVIIPRGADNLVAINLIVQHIQDILNGGPSKRQTNGCLNGYTPSRK RQASESSSRPHLEHHHHHH |
| 预测分子量 | 30 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UCK2重组蛋白的3篇参考文献示例(内容为虚构，仅供格式参考)：
1. **文献名称**：Recombinant human UCK2: expression, purification, and enzymatic characterization
**作者**：Chen L, Wang H, et al.
**摘要**：研究报道了人源UCK2基因的克隆、重组蛋白在大肠杆菌中的表达及纯化方法，并分析了其激酶活性及底物特异性，为核苷类似物药物研发提供基础。
2. **文献名称**：UCK2 regulates nucleotide metabolism and promotes cancer cell proliferation
**作者**：Zhang X, Liu Y, et al.
**摘要**：通过重组UCK2蛋白功能实验，发现其通过调控嘧啶核苷酸合成通路影响肿瘤细胞增殖，提示UCK2可能作为癌症治疗的潜在靶点。
3. **文献名称**：Structural insights into UCK2-inhibitor interactions by crystallography
**作者**：Fang J, Zhou R, et al.
**摘要**：解析了重组UCK2蛋白与抑制剂复合物的晶体结构，揭示了关键结合位点，为设计高选择性抗病毒药物提供结构生物学依据。
注：以上文献信息为示例性虚构内容，实际文献需通过学术数据库检索确认。
**Background of UCK2 Recombinant Protein**
Uridine Cytidine Kinase 2 (UCK2) is a key enzyme in pyrimidine nucleotide metabolism, catalyzing the phosphorylation of uridine and cytidine to their respective monophosphates (UMP and CMP). This reaction is critical for the salvage pathway of nucleotide synthesis, supporting DNA/RNA biosynthesis and cellular proliferation. UCK2 is predominantly expressed in proliferating tissues and has been implicated in pathological conditions, including cancer, where its upregulation correlates with tumor growth and chemoresistance.
Recombinant UCK2 protein is produced using molecular cloning techniques, often in *E. coli* or eukaryotic expression systems, to ensure high purity and enzymatic activity. This engineered protein retains the functional domains necessary for substrate binding and catalysis, enabling studies on its kinetic properties, regulatory mechanisms, and interactions with inhibitors. Researchers utilize UCK2 recombinant protein to explore its role in nucleotide metabolism dysregulation, particularly in cancers reliant on salvage pathways for rapid nucleotide synthesis.
Additionally, UCK2 is a potential therapeutic target. Inhibitors targeting UCK2 activity are being investigated for anticancer therapies, as blocking nucleotide salvage could impair cancer cell survival. Structural studies using recombinant UCK2. such as X-ray crystallography or cryo-EM, provide insights into its conformational dynamics and aid in structure-based drug design. Challenges in its application include maintaining post-translational modifications critical for function, which may require mammalian expression systems. Overall, UCK2 recombinant protein serves as a vital tool for both basic research and translational studies in oncology and metabolic disorders.
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