| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CHEK2; CDS1; CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2 |
| Entrez GeneID | 11200 |
| WB Predicted band size | Calculated MW: 61 kDa; Observed MW: 61 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human Chk2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是关于Chk2抗体的3篇参考文献及其摘要(另附1篇综述供参考)：
---
1. **文献名称**：*Regulation of Chk2 kinase by autoinhibition and ATM-mediated phosphorylation*
**作者**：Hirao A., et al.
**摘要**：该研究通过Western blot和免疫沉淀实验，利用Chk2抗体揭示了ATM激酶对Chk2的磷酸化调控机制，证实Chk2在DNA损伤应答中被激活并参与细胞周期检查点调控。
---
2. **文献名称**：*Substrate specificities and identification of putative substrates of ATM kinase family members*
**作者**：Matsuoka S., et al.
**摘要**：作者使用特异性Chk2抗体进行免疫共沉淀实验，分析了Chk2与ATM激酶的相互作用，并鉴定其底物，阐明其在DNA损伤信号通路中的关键作用。
---
3. **文献名称**：*Phosphorylation of threonine 68 promotes oligomerization and autophosphorylation of the Chk2 protein kinase*
**作者**：Ahn J., et al.
**摘要**：该研究开发了针对Chk2 Thr68磷酸化位点的特异性抗体，验证了其在电离辐射后的激活机制，表明磷酸化依赖的寡聚化是Chk2功能的核心步骤。
---
**补充综述**：
4. **文献名称**：*Chk1 and Chk2 kinases in checkpoint control and cancer*
**作者**：Bartek J., Lukas J.
**摘要**：综述总结了Chk2的调控机制及其在癌症中的作用，引用了多篇基于Chk2抗体的实验(如免疫荧光、Western blot)以支持其在DNA修复中的功能。
---
这些文献涵盖了Chk2抗体的实验应用(如检测表达、磷酸化及相互作用)，可作为相关研究的参考。如需具体实验细节，建议通过数据库(如PubMed)检索全文。
The Chk2 antibody is a crucial tool in studying the checkpoint kinase 2 (Chk2) protein, a serine/threonine kinase central to DNA damage response pathways. Chk2 is activated primarily by the ATM kinase in response to double-strand DNA breaks, leading to cell cycle arrest, DNA repair, or apoptosis. It phosphorylates downstream targets like p53. BRCA1. and Cdc25. regulating genomic stability and tumor suppression. Dysregulation of Chk2 is linked to cancer development and chemotherapy resistance.
Chk2 antibodies are widely used in research to detect Chk2 expression, phosphorylation status (e.g., at Thr68 during activation), and cellular localization via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They help investigate Chk2's role in cancer biology, radiation response, and replication stress. Both monoclonal and polyclonal variants exist, with specificity validated through knockout controls or siRNA knockdown. Some antibodies distinguish between phosphorylated and non-phosphorylated forms, enabling studies of Chk2 activation dynamics. Commercial Chk2 antibodies often undergo rigorous validation using recombinant proteins or cell lysates. Researchers rely on these reagents to explore Chk2's interplay with other DDR components and its potential as a therapeutic target or biomarker in oncology. Proper antibody selection depends on experimental needs, including species reactivity and post-translational modification focus.
×
