| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBXN2B |
| Uniprot No | Q14CS0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-331aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAEGGGPEPGEQERRSSGPRPPSARDL QLALAELYEDEVKCKSSKSNRPKATVFKSPRTPPQRFYSSEHEYSGLNIV RPSTGKIVNELFKEAREHGAVPLNEATRASGDDKSKSFTGGGYRLGSSFC KRSEYIYGENQLQDVQILLKLWSNGFSLDDGELRPYNEPTNAQFLESVKR GEIPLELQRLVHGGQVNLDMEDHQDQEYIKPRLRFKAFSGEGQKLGSLTP EIVSTPSSPEEEDKSILNAVVLIDDSVPTTKIQIRLADGSRLIQRFNSTH RILDVRNFIVQSRPEFAALDFILVTSFPNKELTDESLTLLEADILNTVLL QQLK |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UBXN2B重组蛋白的3篇参考文献示例(内容基于领域相关研究概括，具体文献需通过学术数据库验证)：
1. **标题**: "UBXN2B interacts with p97/VCP and regulates endoplasmic reticulum-associated degradation"
**作者**: Smith J, et al.
**摘要**: 本研究通过重组UBXN2B蛋白的体外结合实验，证实其与p97/VCP复合物的相互作用，并揭示UBXN2B通过调控内质网相关降解(ERAD)通路影响错误折叠蛋白的清除，为相关疾病机制提供了新见解。
2. **标题**: "Structural insights into UBXN2B-mediated ubiquitin signaling"
**作者**: Yamamoto K, et al.
**摘要**: 利用重组UBXN2B蛋白的晶体结构解析，发现其UBX结构域特异性识别泛素化修饰底物，并阐明其在蛋白酶体依赖和非依赖通路中的双重功能，为靶向泛素信号的治疗策略奠定基础。
3. **标题**: "UBXN2B suppresses tumor growth by destabilizing oncogenic transcription factors"
**作者**: Chen L, et al.
**摘要**: 通过重组UBXN2B蛋白的功能实验，证明其通过招募E3泛素连接酶促进致癌转录因子(如c-Myc)的泛素化降解，抑制肿瘤细胞增殖，提示UBXN2B作为潜在抑癌因子的作用机制。
**注意**：以上文献信息为示例，实际研究请通过PubMed、Google Scholar等平台以“UBXN2B recombinant protein”或“UBXN2B function”为关键词检索最新论文。
UBXN2B (UBX Domain Protein 2B) is a member of the ubiquitin regulatory X (UBX) domain-containing protein family, which plays diverse roles in cellular processes such as protein quality control, endoplasmic reticulum-associated degradation (ERAD), and modulation of ubiquitin-dependent signaling pathways. The protein is characterized by its UBX domain, a conserved ~80-amino acid motif that mediates interactions with the AAA-ATPase p97 (VCP), a central regulator of ubiquitin-proteasome system (UPS) functions. UBXN2B also contains a C-terminal domain implicated in binding ubiquitinated substrates or cofactors, positioning it as a potential adaptor molecule in p97-mediated processes.
Recombinant UBXN2B proteins are engineered to facilitate structural and functional studies. These proteins are typically expressed in bacterial (e.g., *E. coli*) or mammalian systems with affinity tags (e.g., His-tag, GST) for purification. Researchers utilize recombinant UBXN2B to investigate its role in regulating p97-dependent pathways, including extraction of misfolded proteins from membranes, cell cycle control, and NF-κB signaling. Notably, UBXN2B has been linked to negative regulation of the NLRP3 inflammasome, suggesting therapeutic relevance in inflammatory diseases. Its interaction with ubiquitin chains and p97 also makes it a target for studying cancer biology, neurodegenerative disorders, and viral infection mechanisms where UPS dysfunction occurs. Recent studies highlight UBXN2B’s involvement in apoptosis regulation through caspase-8 binding, expanding its functional scope. The availability of recombinant UBXN2B enables biochemical assays (e.g., pull-downs, ubiquitination assays), structural analyses (X-ray crystallography, cryo-EM), and drug screening for pathologies linked to p97-UBXN2B dysregulation.
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