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产品描述：豚鼠肿瘤坏死因子α(TNF-α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNF含量。肿瘤坏死因子（TNF）是一种重要的细胞因子。其背景上，它在免疫调节、炎症反应等生理过程中有关键作用。研究机制方面，TNF可通过与受体结合，激活多条信号通路，进而调控细胞的存活、凋亡及炎症因子释放等，在多种疾病发病机制中扮演重要角色。试剂盒检测范围为3.12 pg/mL-200 pg/mL，该产品适用于基础科研中炎症机制研究、药物干预效果评价以及豚鼠模型的免疫相关疾病实验，可通过检测组织匀浆中的 TNF-α 水平分析局部炎症反应，或利用血液样本评估系统性免疫激活状态，其表达水平的变化常用于评估感染性疾病、自身免疫反应或肿瘤研究中的免疫状态。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TNF ELISA Kit; TNFA ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
缩写：TNF
Uniprot No.：P51435
种属：Guinea Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 89-100 | |||||
回收率：
| The recovery of guinea pig TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-97 | ||||
| EDTA plasma (n=4) | 97 | 92-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.3502.3762.3632.2001001.8841.9021.8931.730501.3681.3791.3741.211250.9610.9730.9670.80412.50.6640.6810.6730.5106.250.4750.4860.4810.3183.120.3050.3090.3070.14400.1620.1640.163 | ||||||
货期：3-5 working days
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