首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血栓调节蛋白(TM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TM含量。大鼠血栓调节蛋白（TM）是一种由内皮细胞表达的凝血酶结合蛋白，具有抗凝、抑制血小板聚集和纤溶等作用。其表达与深静脉血栓形成（DVT）的病理过程密切相关，可能成为DVT早期诊断和治疗的潜在靶点。试剂盒检测范围为1.56 ng/mL-100 ng/mL，科研人员可将其应用于心血管疾病模型、药物作用机制、内皮细胞功能研究等领域，例如评估病理状态下TM的表达变化或探究干预措施对凝血调控的影响，为体外实验提供可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：TM
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 87-95 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-97 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 94-102 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of rat TM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-97 | ||||
| EDTA plasma (n=4) | 95 | 92-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.8882.8242.8562.689502.2592.2342.2472.080251.4321.4211.4271.26012.50.7770.7890.7830.6166.250.4760.4540.4650.2983.120.3280.3350.3320.1651.560.2460.2370.2420.07500.1690.1650.167 | ||||||
货期：3-5 working days
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