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产品描述：人Toll样受体9(TLR-9/CD289)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TLR9含量。TLR9即Toll样受体9，是天然免疫系统中重要模式识别受体。它可识别病原体相关分子模式，触发免疫信号级联反应。研究聚焦其在自身免疫病、肿瘤等疾病中的作用，通过调控其活性有望为疾病防治提供新思路。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于探究TLR - 9在炎症性疾病模型中的表达动态、评估免疫调节药物对TLR - 9通路的影响，或作为免疫代谢研究中的配套检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD 289 ELISA Kit; CD289 ELISA Kit; TLR 9 ELISA Kit; TLR9 ELISA Kit; TLR9_HUMAN ELISA Kit; Toll like receptor 9 ELISA Kit; Toll like receptor 9 isoform A precursor ELISA Kit; Toll like receptor 9 isoform B ELISA Kit; Toll-like receptor 9 ELISA Kit
缩写：TLR9
Uniprot No.：Q9NR96
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TLR-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 89-97 | |||||
回收率：
| The recovery of human TLR-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 105 | 100-112 | ||||
| EDTA plasma (n=4) | 94 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.6272.5172.5722.47225001.9432.0261.9851.88512501.3391.3021.3211.2216250.7140.7750.7450.645312.50.4050.3840.3950.295156.250.2610.2730.2670.167780.1770.1870.1820.08200.1020.0980.100 | ||||||
货期：3-5 working days
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