Pig thrombomodulin,TM ELISA Kit

货号: IPD-E06530p

Price： ￥3000

48T 96T

数量：

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产品描述：猪血栓调节蛋白(TM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TM含量。猪血栓调节蛋白（TM）是一种重要的凝血调节因子，其通过与凝血酶结合，激活蛋白C，达到抗凝和促纤溶作用。基因工程猪肾脏移植中的分子检测显示，TM的表达有助于防止血栓形成和出血并发症，是异种移植中重要的免疫调节和抗凝因子。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于实验室研究猪心血管疾病模型、凝血功能异常机制、炎症相关病理过程及内皮细胞功能评估等领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：TM
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig TM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	97
	Range %	92-103
1:4	Average %	85
	Range %	81-87
1:8	Average %	89
	Range %	83-96

回收率：

The recovery of pig TM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	110	103-118

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/mlOD1OD2AverageCorrected202.5392.6722.6062.500101.8551.9881.9221.81650.8810.8850.8830.7772.50.3870.4110.3990.2931.250.2490.2550.2520.1460.6250.1680.1780.1730.0670.3120.1400.1410.1410.03500.1030.1080.106

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