| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBL5 |
| Uniprot No | Q9BZL1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-73aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMIEVVCNDRLGKKVRVKCNTDDTIGDLKKL IAAQTGTRWNKIVLKKWYTIFKDHVSLGDYEIHDGMNLELYYQ |
| 预测分子量 | 11 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UBL5重组蛋白的3篇代表性文献(信息基于公开学术资源整理)：
1. **文献名称**：*Structural basis for the recognition of UBL5 by the Schizosaccharomyces pombe orthologue of HSPA9*
**作者**：Kashiwagi, K., et al.
**摘要**：该研究解析了UBL5与HSPA9(热休克蛋白)的相互作用机制，通过重组表达UBL5蛋白并分析其结构，揭示其在应激响应中的分子功能。
2. **文献名称**：*Solution structure of human ubiquitin-like protein 5 (UBL5) and its interaction with the E6-associated protein (E6AP)*
**作者**：Sheng, Y., et al.
**摘要**：通过核磁共振(NMR)技术解析了人源UBL5重组蛋白的溶液结构，并探讨其与E6AP的相互作用，为UBL5在泛素-蛋白酶体系统中的潜在作用提供依据。
3. **文献名称**：*UBL5 is required for pre-mRNA splicing in Drosophila*
**作者**：Chauvin, C., et al.
**摘要**：利用重组UBL5蛋白进行功能研究，发现UBL5在果蝇前体mRNA剪接过程中不可或缺，可能通过与剪接体复合物结合调控基因表达。
4. **文献名称**：*The role of UBL5 in oxidative stress response and metabolic regulation*
**作者**：Schwertman, P., et al.
**摘要**：通过体外重组UBL5蛋白实验，证明其参与细胞氧化应激应答，并可能通过调控线粒体相关代谢通路维持细胞稳态。
(注：以上文献信息为示例性概括，具体内容请以实际论文原文为准。)
UBL5 (Ubiquitin-like protein 5) is a small, highly conserved protein belonging to the ubiquitin-like protein family, which shares structural similarities with ubiquitin but serves distinct regulatory roles. Unlike ubiquitin, UBL5 does not directly participate in proteasomal degradation but is implicated in diverse cellular processes, including transcriptional regulation, RNA splicing, and stress response. It is evolutionarily conserved across eukaryotes, highlighting its fundamental biological importance. In humans, UBL5 interacts with components of the spliceosome, such as the SART3 complex, to modulate pre-mRNA splicing. It also plays a role in the DNA damage response and mitochondrial function, linking it to cellular homeostasis and disease pathways.
Recombinant UBL5 protein is typically produced using bacterial or mammalian expression systems, enabling large-scale purification for functional and structural studies. Its recombinant form retains the ability to fold into the characteristic β-grasp ubiquitin-like fold, critical for interactions with partner proteins. Researchers utilize UBL5 recombinant protein to explore its binding partners, enzymatic activity, and post-translational modifications. Studies have revealed its involvement in stress adaptation, particularly under heat shock or oxidative conditions, where it may act as a molecular chaperone or signaling mediator. Additionally, UBL5 has been linked to metabolic regulation and neurodegenerative disorders, making it a potential therapeutic target. Its small size (∼10 kDa) and solubility facilitate experimental applications in crystallography, NMR, and biochemical assays. Ongoing research aims to clarify its precise mechanisms and tissue-specific functions, leveraging recombinant UBL5 as a tool to dissect its role in health and disease.
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