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产品描述：人toll样受体4(TLR4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TLR4含量。TLR4（Toll样受体4）是一种模式识别受体，主要识别病原体相关分子模式（PAMPs），如细菌脂多糖（LPS）。其研究机制涉及TLR4与PAMPs结合后，通过MyD88等接头蛋白激活NF-κB，进而诱导炎症反应和免疫应答。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外研究TLR4在炎症反应中的调控机制、药物干预对免疫通路的影响，或分析疾病模型中TLR4水平与病理进程的关联性。实验兼容多种生物样本类型，包括临床前研究的动物模型血清、细胞刺激实验后的培养上清，以及经标准化处理的组织裂解液，为免疫学、肿瘤学等基础研究提供高效检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ARMD10 ELISA Kit; CD284 ELISA Kit; CD284 antigen ELISA Kit; ESOP 1 ELISA Kit; ESOP1 ELISA Kit; Homolog of Drosophila toll ELISA Kit; hToll ELISA Kit; LY 96 ELISA Kit; LY96 ELISA Kit; Lymphocyte antigen 96 ELISA Kit; md 2 ELISA Kit; MD 2 protein ELISA Kit; MD2 protein ELISA Kit; Myeloid differentiation protein 2 ELISA Kit; Protein MD 2 ELISA Kit; Protein MD2 ELISA Kit; TLR 4 ELISA Kit; TLR4 ELISA Kit; TLR4_HUMAN ELISA Kit; TOLL ELISA Kit; Toll like receptor 4 ELISA Kit; Toll-like receptor 4 ELISA Kit
缩写：TLR4
Uniprot No.：O00206
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TLR4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 85 | ||||
| Range % | 80-93 | |||||
| 1:10 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:20 | Average % | 97 | ||||
| Range % | 93-104 | |||||
| 1:40 | Average % | 95 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of human TLR4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 89-103 | ||||
| EDTA plasma (n=4) | 97 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.4632.5472.5052.37252.1212.1722.1472.0142.51.5401.5161.5281.3951.250.7250.7470.7360.6030.6250.4180.4360.4270.2940.3120.2360.2410.2390.1060.1560.1990.2060.2030.07000.1320.1340.133 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human TLR4 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled TLR4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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