Mouse Thrombopoietin,TPO ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E04746m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠血小板生成素(TPO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的THPO含量。THPO即血小板生成素，作为调节血小板生成的关键因子，具有刺激巨核细胞生长、分化和促进血小板生成的重要作用。其研究机制主要围绕与受体结合激活信号通路展开，为血小板减少症等相关疾病的治疗药物研发提供了重要靶点。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于探究TPO在血液系统疾病模型中的动态表达规律、评估药物干预对血小板生成的影响，或解析组织微环境中TPO的调控机制等科研领域，为研究血小板生成调控网络提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Thpo ELISA Kit; Thrombopoietin ELISA Kit; C-mpl ligand ELISA Kit; ML ELISA Kit; Megakaryocyte colony-stimulating factor ELISA Kit; Megakaryocyte growth and development factor ELISA Kit; MGDF ELISA Kit; Myeloproliferative leukemia virus oncogene ligand ELISA Kit
缩写：
THPO
Uniprot No.：
P40226
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
6.25 pg/mL-400 pg/mL
灵敏度：
1.56 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-100
1:2	Average %	93
	Range %	88-97
1:4	Average %	93
	Range %	86-98
1:8	Average %	90
	Range %	88-96

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
400	2.540	2.634	2.587	2.409
200	1.857	1.767	1.812	1.634
100	1.120	1.128	1.124	0.946
50	0.734	0.729	0.732	0.553
25	0.501	0.511	0.506	0.328
12.5	0.369	0.353	0.361	0.183
6.25	0.272	0.284	0.278	0.100
0	0.182	0.175	0.178