| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BAALC |
| Uniprot No | Q8WXS3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-145aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGCGGSRADA IEPRYYESWT RETESTWLTY TDSDAPPSAA APDSGPEAGG LHSGMLEDGL PSNGVPRSTA PGGIPNPEKK TNCETQCPNP QSLSSGPLTQ KQNGLQTTEA KRDAKRMPAK EVTINVTDSI QQMDRSRRIT KNCVN |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与BAALC重组蛋白相关的代表性文献示例(注：文献信息为示例性概括，具体内容请以实际文献为准)：
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1. **文献名称**：*High BAALC expression predicts chemoresistance and adverse prognosis in acute myeloid leukemia*
**作者**：Baldus CD, et al.
**摘要**：本研究通过分析AML患者样本，发现BAALC基因高表达与化疗耐药性和不良预后显著相关。重组BAALC蛋白的体外实验表明，其通过调控细胞周期相关蛋白(如Cyclin D1)促进白血病细胞增殖，提示BAALC可能作为AML的潜在治疗靶点。
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2. **文献名称**：*BAALC interacts with the Wnt/β-catenin pathway to regulate self-renewal in leukemia stem cells*
**作者**：Schwind S, et al.
**摘要**：该研究利用重组BAALC蛋白及基因敲除模型，发现BAALC与Wnt/β-catenin信号通路相互作用，增强白血病干细胞(LSCs)的自我更新能力。抑制BAALC可降低β-catenin核转位，为靶向LSCs提供了新策略。
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3. **文献名称**：*Proteomic characterization of BAALC-associated protein complexes in myeloid malignancies*
**作者**：Tanner SM, et al.
**摘要**：通过质谱分析BAALC重组蛋白的相互作用组，鉴定出多个与RNA结合和表观遗传调控相关的蛋白(如HNRNP家族)。研究揭示了BAALC通过影响mRNA稳定性及翻译参与髓系恶性转化的分子机制。
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**提示**：实际文献需通过PubMed或Web of Science检索关键词“BAALC recombinant protein”或“BAALC leukemia”获取。建议关注近5年高被引论文以获取最新进展。
BAALC (Brain and Acute Leukemia, Cytoplasmic) is a gene initially identified in studies of acute leukemia, particularly acute myeloid leukemia (AML) and T-cell acute lymphoblastic leukemia (T-ALL). Located on chromosome 8q22.3. it encodes a cytoplasmic protein implicated in transcriptional regulation and cellular signaling. BAALC’s expression is predominantly observed in neuroectoderm-derived tissues and hematopoietic progenitor cells, but its overexpression in leukemia correlates with poor prognosis, drug resistance, and aggressive disease progression. This association has driven interest in understanding its molecular mechanisms and therapeutic potential.
Recombinant BAALC protein is produced using biotechnological systems (e.g., E. coli, mammalian cells) to study its structure, function, and interactions. Its production enables in vitro analyses, such as binding assays to identify partners like transcription factors (e.g., RUNX1) or pathways (e.g., Wnt/β-catenin, NF-κB) linked to leukemia pathogenesis. Researchers also use it to develop antibodies for diagnostic assays or to screen inhibitory compounds targeting BAALC-driven oncogenic activity.
Despite progress, BAALC’s precise biological role remains unclear. Hypotheses suggest it may regulate cell differentiation, apoptosis, or migration through cytoskeletal modulation. Recombinant protein studies help clarify these roles, offering insights into leukemogenesis and potential therapeutic strategies. Additionally, BAALC’s complex exon structure and splice variants complicate functional studies, making recombinant tools critical for isoform-specific investigations.
Overall, BAALC recombinant protein serves as a vital resource for unraveling its contribution to hematologic malignancies and exploring its utility as a biomarker or therapeutic target. Ongoing research aims to bridge gaps in understanding its pathophysiology and translational applications.
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