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Rabbit Monoclonal BTF3 Antibody

  • 中文名： BTF3抗体
  • 别    名： NACB; BTF3a; BTF3b; BETA-NAC
货号: IPDX21140
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/20 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesNACB; BTF3a; BTF3b; BETA-NAC
Entrez GeneID689
WB Predicted band sizeCalculated MW: 22 kDa; Observed MW: 22 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenRecombinant protein of human BTF3
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于BTF3抗体的虚构参考文献示例(内容为模拟生成，非真实文献)：

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1. **文献名称**: "BTF3 regulates apoptosis in colorectal cancer via modulating Bcl-2 family proteins"

**作者**: Zhang L, et al.

**摘要**: 本研究利用BTF3特异性抗体，通过免疫沉淀和Western blot技术，发现BTF3通过调控Bcl-2和Bax蛋白表达影响结直肠癌细胞凋亡。抗体验证表明BTF3在肿瘤组织中高表达并与不良预后相关。

2. **文献名称**: "Development of a monoclonal antibody against human BTF3 for diagnostic applications"

**作者**: Kim S, et al.

**摘要**: 文章报道了一种高特异性抗BTF3单克隆抗体的制备与验证。该抗体在ELISA和免疫组化中表现优异，成功应用于肺癌组织样本的BTF3蛋白水平检测，证实其作为潜在生物标志物的价值。

3. **文献名称**: "BTF3 antibody-based profiling reveals its role in embryonic development"

**作者**: Müller R, et al.

**摘要**: 通过BTF3抗体进行小鼠胚胎组织的免疫荧光分析，发现BTF3在神经管发育和器官形成中动态表达，提示其在细胞分化和转录调控中的关键作用。抗体特异性经基因敲除实验验证。

4. **文献名称**: "BTF3 interacts with RNA polymerase II: Evidence from co-immunoprecipitation assays"

**作者**: Chen H, et al.

**摘要**: 使用BTF3抗体进行共免疫沉淀实验，证实BTF3与RNA聚合酶II的亚基直接结合，参与转录起始复合物的组装。研究为BTF3在基础转录机制中的功能提供了实验支持。

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注：以上文献信息为模拟生成，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。

背景信息

The BTF3 (Basic Transcription Factor 3) antibody targets a protein component of the NC2 (Negative Cofactor 2) complex, which plays a dual role in transcriptional regulation. BTF3. also known as NC2β, forms a heterodimer with NC2α to modulate RNA polymerase II-mediated transcription. It represses basal transcription by binding to TATA-box-binding protein (TBP) and blocking preinitiation complex assembly, while also enhancing activated transcription in certain contexts. BTF3 is ubiquitously expressed and evolutionarily conserved, underscoring its fundamental role in cellular processes. Studies link BTF3 to cell cycle progression, apoptosis, and differentiation, with dysregulation observed in cancers such as neuroblastoma and breast cancer. Antibodies against BTF3 are widely used in research to investigate its expression patterns, subcellular localization, and interaction partners via techniques like Western blotting, immunohistochemistry, and co-immunoprecipitation. These tools have helped elucidate BTF3's involvement in oncogenic pathways and its potential as a diagnostic or prognostic biomarker. Recent work also explores its role in developmental biology and response to DNA damage. Commercial BTF3 antibodies are typically raised in rabbits or mice, validated for specificity across human and model organisms.

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