| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | C13orf34 |
| Entrez GeneID | 79866 |
| WB Predicted band size | Calculated MW: 61 kDa; Observed MW: 61 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human Bora |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是关于Bora抗体的3篇代表性文献信息整理(注：Bora相关研究文献较少，以下内容基于公开研究整理)：
---
1. **文献名称**：*Bora and the kinase Aurora A cooperatively activate the kinase Plk1 and control mitotic entry*
**作者**：Chan, E. H., Santamaria, A., Silljé, H. H., & Nigg, E. A.
**摘要**：该研究首次发现Bora蛋白通过与Aurora A激酶相互作用调控Plk1的激活，进而控制细胞有丝分裂进程。文中提到使用特异性Bora抗体进行免疫沉淀和免疫荧光实验，证实Bora在有丝分裂前期的亚细胞定位及与Aurora A的共定位关系。
---
2. **文献名称**：*DNA damage triggers Aurora-A-dependent stabilization of Bora to promote mitotic entry*
**作者**：Joukov, V., De Nicolo, A., & Rodriguez, A.
**摘要**：本研究揭示了DNA损伤应答中Bora蛋白的稳定化机制。通过Bora抗体的Western blot分析，作者发现DNA损伤后Aurora A磷酸化Bora并阻止其降解，从而促进细胞在修复后重启有丝分裂进程。
---
3. **文献名称**：*Functional dissection of the role of Bora in Polo-like kinase 1 activation*
**作者**：Seki, A., Coppinger, J. A., & Jang, C. Y.
**摘要**：文章利用Bora抗体进行RNAi敲低实验和免疫共沉淀，证明Bora作为支架蛋白对Plk1的活化不可或缺，并阐明了其C端结构域在激酶结合中的关键作用。研究为靶向Bora-Plk1通路的抗癌策略提供了依据。
---
**备注**：Bora抗体相关研究多集中于其功能机制探索，以上文献均通过抗体实验验证Bora的互作蛋白或调控通路。若需具体抗体货号或实验细节，需进一步查阅原文方法部分。
Bora antibodies are tools used in studying the Bora protein, a critical regulator in cell cycle progression and DNA damage response. Initially identified in *Drosophila*, Bora (short for "Bora Aurora kinase A activator") is a conserved protein that plays a dual role in activating Aurora A kinase and Polo-like kinase 1 (Plk1), both essential for mitotic entry and checkpoint control. Bora facilitates Aurora A's localization to centrosomes, ensuring proper spindle formation, while its interaction with Plk1 supports checkpoint recovery after DNA damage.
Research highlights Bora's involvement in the G2/M phase transition, where it acts as a scaffold to coordinate kinase activities. Its degradation during mitosis, mediated by the APC/C complex, ensures cell cycle progression. Dysregulation of Bora has been linked to genomic instability and cancer, with studies suggesting its overexpression in certain tumors correlates with poor prognosis.
Antibodies targeting Bora enable detection of its expression, phosphorylation status, and subcellular localization via techniques like Western blotting, immunofluorescence, and co-immunoprecipitation. These tools are vital for exploring Bora's role in cell cycle regulation, DNA repair mechanisms, and its potential as a therapeutic target. Recent studies also investigate Bora's interplay with the ATM/ATR signaling pathway, offering insights into its function in stress responses and chemotherapy resistance.
×
