首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠酪氨酸羟化酶(TH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织匀浆样本中的TH含量。试剂盒检测范围为78 pg/mL-5000 pg/mL，科研人员可通过定量分析大鼠中枢神经系统、肾上腺等组织或外周血中TH的动态变化，用于探究神经退行性疾病模型、应激反应机制、药物干预效果等研究，为神经生物学、药理学领域的分子机制研究提供可靠工具。本产品严格限定于科学研究用途，实验结果不作为任何医疗决策依据。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Th ELISA Kit; Tyrosine 3-monooxygenase ELISA Kit; EC 1.14.16.2 ELISA Kit; Tyrosine 3-hydroxylase ELISA Kit; TH ELISA Kit
缩写：TH
Uniprot No.：P04177
种属：Rattus norvegicus (Rat)
样本类型：serum, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 91-105 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rat TH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.5632.5012.5322.39025002.1132.1312.1221.98012501.6831.6991.6911.5496251.1421.1681.1551.0133120.7120.7540.7330.5911560.4180.4310.4250.283780.3220.3410.3320.19000.1410.1430.142 | ||||||
货期：3-5 working days
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