| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBE2R2 |
| Uniprot No | Q712K3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-238aa |
| 氨基酸序列 | AQQQMTSSQKALMLELKSLQEEPVEGFRITLVDESDLYNWEVAIFGPPNT LYEGGYFKAHIKFPIDYPYSPPTFRFLTKMWHPNIYENGDVCISILHPPV DDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMFRKW RDSKGKDKEYAEIIRKQVSATKAEAEKDGVKVPTTLAEYCIKTKVPSNDN SSDLLYDDLYDDDIDDEDEEEEDADCYDDDDSGNEES |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UBE2R2重组蛋白的3篇参考文献的简要信息，基于公开研究领域相关主题的典型文献结构概括(注：部分内容为示例性模拟，实际文献需根据具体论文调整)：
1. **文献名称**: "UBE2R2 regulates the ubiquitin-mediated degradation of p53 and is critical for tumor progression"
**作者**: Li X, Chen Y, et al.
**摘要**: 该研究揭示了UBE2R2作为泛素结合酶，通过介导p53蛋白的泛素化修饰促进其蛋白酶体降解，从而影响肿瘤细胞的增殖和凋亡。重组UBE2R2蛋白在体外实验中证实了其与MDM2的相互作用及对p53稳定性的调控作用。
2. **文献名称**: "Structural characterization of recombinant UBE2R2 and its interaction with E3 ligases"
**作者**: Smith J, Wang H, et al.
**摘要**: 作者通过X射线晶体学解析了重组UBE2R2蛋白的三维结构，并鉴定了其与多种E3泛素连接酶(如APC/C)结合的关键结构域。研究为UBE2R2在细胞周期调控中的功能提供了结构基础。
3. **文献名称**: "UBE2R2-ASK1 complex promotes oxidative stress-induced apoptosis via JNK signaling"
**作者**: Tanaka K, Suzuki T, et al.
**摘要**: 该研究利用重组UBE2R2蛋白进行体外互作实验，发现其与凋亡信号调节激酶ASK1的直接结合，并通过增强JNK通路的激活参与氧化应激诱导的细胞凋亡，提示UBE2R2在神经退行性疾病中的潜在作用。
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**注意**：以上文献信息为模拟示例，实际文献需通过PubMed、Web of Science等平台检索。如需真实文献，建议使用关键词“UBE2R2 recombinant”“UBE2R2 ubiquitination”进行查询，并筛选近年的功能或结构研究论文。
**Background of UBE2R2 Recombinant Protein**
UBE2R2 (Ubiquitin-Conjugating Enzyme E2 R2) is a member of the E2 ubiquitin-conjugating enzyme family, central to the ubiquitin-proteasome system (UPS), which regulates protein degradation, cell cycle progression, DNA repair, and signal transduction. As an E2 enzyme, UBE2R2 facilitates the transfer of ubiquitin from E1-activating enzymes to substrate proteins, often in partnership with E3 ubiquitin ligases like the SCF (SKP1-CUL1-F-box) complex. This post-translational modification typically marks target proteins for proteasomal degradation or alters their functional states.
The recombinant UBE2R2 protein is engineered *in vitro* using expression systems such as *E. coli* or mammalian cells, ensuring high purity and activity for experimental applications. It retains the conserved catalytic core structure of E2 enzymes, including a critical cysteine residue required for thioester bond formation with ubiquitin. Recombinant variants may include affinity tags (e.g., His-tag) for simplified purification and detection.
Functionally, UBE2R2 is implicated in cell cycle regulation, particularly the G1/S transition, by mediating the degradation of cyclin-dependent kinase inhibitors. Dysregulation of UBE2R2 is linked to cancers, neurodegenerative diseases, and developmental disorders, highlighting its therapeutic relevance. Researchers employ recombinant UBE2R2 to study ubiquitination mechanisms, screen for modulators of UPS activity, or explore pathological pathways in disease models.
Its recombinant form offers advantages over native protein isolation, providing consistent quality, scalability, and reduced batch variability. Studies leveraging UBE2R2 recombinant protein contribute to understanding UPS dynamics and developing targeted therapies, underscoring its value in both basic research and drug discovery.
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