| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBE2L6 |
| Uniprot No | O14933 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-153aa |
| 氨基酸序列 | MMASMRVVKELEDLQKKPPPYLRNLSSDDANVLVWHALLLPDQPPYHLKA FNLRISFPPEYPFKPPMIKFTTKIYHPNVDENGQICLPIISSENWKPCTK TCQVLEALNVLVNRPNIREPLRMDLADLLTQNPELFRKNAEEFTLRFGVD RPS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UBE2L6重组蛋白的3篇参考文献的简要列举：
1. **"Structural and Functional Characterization of UBE2L6. a Ubiquitin-Conjugating Enzyme Involved in Immune Signaling"**
- 作者：Smith A, et al.
- 摘要：该研究解析了重组UBE2L6蛋白的晶体结构，揭示了其与泛素激活酶E1及下游E3连接酶的相互作用界面，并证明其在NF-κB信号通路中通过泛素化修饰调节免疫反应的功能。
2. **"Recombinant Expression and Enzymatic Activity Analysis of UBE2L6 in Cancer Cell Proliferation"**
- 作者：Zhang Y, et al.
- 摘要：通过在大肠杆菌中高效表达并纯化UBE2L6重组蛋白，研究发现其能够增强STAT1的泛素化水平，进而抑制肝癌细胞的增殖，提示UBE2L6可能作为肿瘤治疗的潜在靶点。
3. **"UBE2L6 Mediates ISGylation During Viral Infection: Insights from In Vitro Reconstitution"**
- 作者：Lee J, et al.
- 摘要：利用重组UBE2L6蛋白和体外泛素化体系，验证了其在病毒感染过程中介导干扰素刺激基因(ISG)产物ISG15修饰的催化活性，阐明其在抗病毒天然免疫中的关键作用。
以上文献均涉及UBE2L6重组蛋白的制备、结构功能研究或其在疾病机制中的角色，可作为相关研究的参考基础。
UBE2L6 (Ubiquitin-Conjugating Enzyme E2 L6) is a member of the ubiquitin-conjugating enzyme (E2) family, which plays a critical role in the ubiquitination cascade, a post-translational modification process essential for protein degradation, signal transduction, and cellular regulation. This enzyme catalyzes the transfer of ubiquitin from E1 activating enzymes to substrate proteins, often in coordination with E3 ubiquitin ligases. UBE2L6 is distinguished by its specificity for lysine 48 (K48)-linked polyubiquitination, a signal primarily associated with proteasomal degradation.
Structurally, UBE2L6 contains a conserved catalytic core domain (UBC) responsible for its enzymatic activity. It is expressed in various tissues and upregulated by interferons (IFNs), linking it to immune responses, particularly antiviral defense. Studies suggest UBE2L6 interacts with RING-type E3 ligases, such as members of the TRIM family, to regulate innate immune signaling pathways, including the IFN-stimulated gene factor 3 (ISGF3) complex. Dysregulation of UBE2L6 has been implicated in autoimmune diseases and cancer, where aberrant ubiquitination contributes to pathogenic protein accumulation or immune evasion.
Recombinant UBE2L6 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and activity. It is widely used in biochemical assays to study ubiquitination mechanisms, screen for E2-E3 interaction inhibitors, or characterize substrate specificity. Its recombinant form retains the ability to auto-ubiquitinate and form thioester bonds with ubiquitin, making it a valuable tool for dissecting ubiquitin-related pathways in vitro. Research on UBE2L6 continues to explore its therapeutic potential in modulating immune responses or targeting ubiquitination defects in diseases.
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