| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | B9D2 |
| Uniprot No | Q9BPU9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-175aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAEVHVIGQIIGASGFSESSLFCKWGI HTGAAWKLLSGVREGQTQVDTPQIGDMAYWSHPIDLHFATKGLQGWPRLH FQVWSQDSFGRCQLAGYGFCHVPSSPGTHQLACPTWRPLGSWREQLARAF VGGGPQLLHGDTIYSGADRYRLHTAAGGTVHLEIGLLLRNFDRYGVEC |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于B9D2重组蛋白的参考文献示例(注：以下内容为假设性示例，实际文献需通过学术数据库核实)：
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1. **文献名称**：*"Functional Analysis of B9D2 Recombinant Protein in Ciliogenesis"*
**作者**：Smith J, Lee K, et al.
**摘要**：本研究通过在大肠杆菌中表达并纯化B9D2重组蛋白，揭示了其在纤毛形成中的关键作用。实验表明，B9D2与CEP290蛋白相互作用，调控纤毛基体的组装，为相关纤毛疾病的机制提供了新见解。
2. **文献名称**：*"Structural Characterization of Recombinant B9D2 and Its Role in Meckel Syndrome"*
**作者**：Garcia R, Zhang Y, et al.
**摘要**：利用X射线晶体学解析了B9D2重组蛋白的三维结构，发现其B9结构域与细胞膜结合相关。功能实验证实，B9D2突变体导致纤毛功能障碍，与Meckel综合征患者的表型密切相关。
3. **文献名称**：*"Development of a High-Yield Expression System for B9D2 Recombinant Protein in Mammalian Cells"*
**作者**：Chen L, Wang H, et al.
**摘要**：优化了B9D2在哺乳动物细胞中的重组表达条件，获得高纯度蛋白用于抗体生产。该方法为基于B9D2的分子诊断工具和靶向治疗研究奠定了基础。
4. **文献名称**：*"B9D2 Recombinant Protein Interacts with Ciliary Transport Complexes in vitro"*
**作者**：Johnson M, Patel S, et al.
**摘要**：通过体外结合实验，证明重组B9D2蛋白与IFT-A复合体成员直接互作，支持其在纤毛内运输中的调控功能，为纤毛相关信号通路研究提供了实验依据。
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**注意**：上述文献为示例性内容，实际研究中请通过**PubMed**、**Google Scholar**或**Web of Science**等平台，以关键词“B9D2 recombinant protein”“B9D2 cilia”“B9D2 disease”检索最新文献。真实研究可能集中于B9D2的病理机制、结构功能或重组表达技术优化等领域。
**Background of B9D2 Recombinant Protein**
B9D2. also known as B9 domain-containing protein 2. is a member of the B9 protein family, which is evolutionarily conserved and implicated in ciliogenesis and cellular signaling pathways. This protein is characterized by the presence of a B9 domain, a structural motif involved in protein-protein interactions. B9D2 plays a critical role in the formation and function of primary cilia, microtubule-based organelles essential for sensing extracellular signals, including Sonic Hedgehog (SHH) signaling. Dysregulation of primary cilia is associated with developmental disorders collectively termed ciliopathies, such as Meckel syndrome (MKS) and Joubert syndrome.
The recombinant B9D2 protein is engineered using genetic recombination technology, typically expressed in prokaryotic (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian cells) to ensure proper folding and post-translational modifications. Recombinant B9D2 serves as a vital tool for studying its molecular interactions, structural properties, and functional roles in ciliary assembly and signaling pathways. Researchers utilize it to investigate disease mechanisms, screen for therapeutic targets, or validate binding partners through techniques like co-immunoprecipitation or surface plasmon resonance.
Studies have shown that B9D2 interacts with other ciliopathy-associated proteins, such as TMEM218 and MKS1. forming complexes critical for ciliary membrane organization. Mutations in *B9D2* are linked to severe ciliopathies, underscoring its biomedical relevance. Recombinant B9D2 production enables functional rescue experiments in disease models and aids in developing diagnostic assays. Its application extends to exploring tissue-specific ciliary functions and potential therapeutic interventions targeting cilia-related disorders.
In summary, B9D2 recombinant protein bridges basic research and clinical insights, offering a platform to dissect ciliary biology and advance understanding of ciliopathies.
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