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产品描述：大鼠末端补体复合物C5b-9(TCC C5b-9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TCC C5b9含量。大鼠末端补体复合物C5b-9（TCC C5b-9）是补体系统激活后形成的膜攻击复合物，通过介导细胞膜穿孔参与免疫防御及炎症反应调控，在免疫相关疾病机制研究中具有重要价值。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于基础医学研究中补体系统功能评估，例如在建立肾小球肾炎、心肌梗死或神经退行性疾病动物模型时，可通过检测血清或组织中的C5b - 9水平动态观察补体激活程度；亦可用于评价药物或生物制剂对补体通路的调控作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：TCC C5b9
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 86-98 | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 92-102 | |||||
| 1:400 | Average % | 85 | ||||
| Range % | 81-88 | |||||
| 1:800 | Average % | 88 | ||||
| Range % | 83-95 | |||||
回收率：
| The recovery of rat TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 90 | 87-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.3512.5022.4272.282502.0212.1652.0931.948251.4231.5401.4821.33712.50.9361.0020.9690.8246.250.6070.5810.5940.4493.120.3720.3600.3660.2211.560.2380.2570.2480.10300.1410.1490.145 | ||||||
货期：3-5 working days
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