| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | APG12-like; APG12L; ATG12; Autophagy 12; Autophagy-related protein 12; FBR93; HAPG12; Ubiquitin-like protein ATG12 |
| Entrez GeneID | 9140 |
| WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human Apg12 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于ATG12抗体的代表性文献，简要整理供参考：
1. **《Autophagy fights disease through cellular self-digestion》**
作者：Mizushima N, Levine B, Cuervo AM, et al.
摘要：综述自噬在疾病中的调控机制，提到ATG12-ATG5结合对自噬体形成的关键作用，并强调ATG12抗体在检测自噬活性中的实验应用。
2. **《The role of autophagy during the early neonatal starvation period》**
作者：Kuma A, Hatano M, Matsui M, et al.
摘要：研究新生小鼠饥饿诱导的自噬，利用ATG12抗体通过免疫印迹验证自噬相关蛋白表达变化，证实ATG12-ATG5复合物对细胞存活的重要性。
3. **《Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy》**
作者：Pattingre S, Tassa A, Qu X, et al.
摘要：探讨Bcl-2与自噬蛋白Beclin1的相互作用，实验中采用ATG12抗体检测自噬通量，揭示凋亡与自噬通路间的交叉调控机制。
4. **《Loss of autophagy in the central nervous system causes neurodegeneration in mice》**
作者：Hara T, Nakamura K, Matsui M, et al.
摘要：通过神经元特异性敲除Atg5/Atg12的小鼠模型，结合ATG12抗体的免疫组化分析，证明自噬缺陷导致神经退行性病变。
注：以上文献为领域内经典研究，实际引用时建议核对最新版本或补充近年研究(如2018年后关于ATG12在癌症免疫中的文献)。
ATG12 antibodies are essential tools for studying the molecular mechanisms of autophagy, a conserved cellular process responsible for degrading damaged organelles and proteins. Autophagy-related gene 12 (ATG12) is a ubiquitin-like protein critical for autophagosome formation. During autophagy initiation, ATG12 undergoes a conjugation process: it is first activated by the E1-like enzyme ATG7. transferred to the E2-like enzyme ATG10. and finally covalently linked to ATG5. The ATG12-ATG5 conjugate interacts with ATG16L1 to form a complex that facilitates the lipidation of LC3 (microtubule-associated protein 1A/1B-light chain 3), a key step in autophagosome membrane expansion.
ATG12 antibodies are widely used in research to detect ATG12 expression levels, conjugation status, and subcellular localization via techniques like Western blotting, immunofluorescence, and immunoprecipitation. These antibodies help elucidate autophagy dysregulation in diseases such as cancer, neurodegenerative disorders, and infections. For example, reduced ATG12-ATG5 conjugation may indicate impaired autophagy, linked to pathological protein aggregation in Alzheimer’s disease. Both monoclonal and polyclonal ATG12 antibodies are available, with specificity validated across species (human, mouse, rat). Researchers also use them to assess autophagy flux when combined with other markers (e.g., LC3-II/p62). Understanding ATG12’s role through antibody-based assays contributes to therapeutic strategies targeting autophagy pathways.
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