| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | APEX1; APE; APE1; APEX; APX; HAP1; REF1; DNA-(apurinic or apyrimidinic site) lyase; APEX nuclease; APEN; Apurinic-apyrimidinic endonuclease 1; AP endonuclease 1; APE-1; REF-1; Redox factor-1 |
| Entrez GeneID | 328 |
| WB Predicted band size | Calculated MW: 36 kDa; Observed MW: 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human APE1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于APE1抗体的参考文献概述：
1. **"APE1/Ref-1 antibody (NB100-116) detects nuclear and cytoplasmic expression in human tissues"**
*作者：Kang et al. (2010)*
摘要：该研究验证了商业抗体NB100-116在免疫组织化学(IHC)和Western blot中对人组织中APE1蛋白的检测特异性，发现其在多种癌症组织中高表达，并提示与化疗耐药性相关。
2. **"Monoclonal antibody-based immunohistochemical analysis of APE1/Ref-1 in glioblastoma multiforme"**
*作者：Bobola et al. (2012)*
摘要：通过单克隆抗体(13B8克隆)检测胶质母细胞瘤中APE1的亚细胞定位，发现其核内高表达与患者预后不良相关，支持APE1作为治疗靶点的潜力。
3. **"Development of a novel APE1-specific monoclonal antibody for DNA repair studies"**
*作者：Fishel et al. (2015)*
摘要：报道了一种新型APE1单克隆抗体(Clone 3A9)的开发，验证了其在流式细胞术和免疫荧光中对细胞核内APE1氧化修复功能的特异性检测，适用于DNA损伤修复机制研究。
4. **"APE1 overexpression promotes ovarian cancer progression via Wnt/β-catenin pathway activation"**
*作者：Wang et al. (2018)*
摘要：使用兔源多克隆抗体(ab194)通过免疫组化分析卵巢癌样本，发现APE1高表达通过激活Wnt通路促进肿瘤侵袭，提示其作为预后标志物的价值。
注：以上文献信息为模拟示例，实际引用需以具体论文内容为准。建议通过PubMed或Web of Science以关键词"APE1 antibody"+"validation"或"application"检索最新研究。
APE1 (apurinic/apyrimidinic endonuclease 1), also known as APEX1 or Ref-1. is a multifunctional enzyme central to DNA repair and redox regulation. Primarily involved in the base excision repair (BER) pathway, APE1 cleaves DNA at abasic sites generated by DNA glycosylases, enabling error-free repair of oxidative or alkylation damage. Beyond its endonuclease activity, APE1 acts as a redox signaling regulator, stabilizing transcription factors like AP-1. NF-κB, and p53 by maintaining their reduced, active states.
APE1 antibodies are essential tools for studying its expression, localization, and function in cellular processes. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate APE1's role in cancer, neurodegenerative diseases, and responses to genotoxic stress. In cancer research, elevated APE1 levels correlate with chemotherapy resistance and poor prognosis, making it a potential therapeutic target.
Commercial APE1 antibodies are typically raised against specific epitopes, with monoclonal and polyclonal variants available. Validation includes testing for cross-reactivity across species (human, mouse, rat) and confirming specificity using knockout controls. Researchers often prioritize antibodies distinguishing between APE1's two functional domains: the N-terminal redox domain and C-terminal repair domain. Proper antibody selection ensures accurate interpretation of APE1's dual roles in genome stability and redox signaling across experimental models.
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