| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | p68; p70; ANX6; CBP68; CPB-II |
| Entrez GeneID | 309 |
| WB Predicted band size | Calculated MW: 76 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human Annexin-6 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Annexin VI抗体的3篇参考文献示例，包含文献名称、作者及摘要概括：
1. **文献名称**：*"Annexin VI modulates calcium-dependent membrane fusion in vitro"*
**作者**：Burgoyne RD, Geisow MJ
**摘要**：该研究利用Annexin VI抗体探究其在体外钙依赖性膜融合中的作用，发现该蛋白通过调节钙离子浓度影响脂质膜重组，提示其可能在胞吐或内吞过程中起调控作用。
2. **文献名称**：*"Subcellular localization of annexin VI in cardiac myocytes: association with sarcolemma and nuclear envelope"*
**作者**：Kaplan R, Jaye M, Burgess WH
**摘要**：通过免疫荧光和Western blot分析，使用特异性抗体证实Annexin VI在心肌细胞中定位于肌膜和核膜，可能参与细胞膜修复或钙信号传导。
3. **文献名称**：*"Annexin VI binds to the GTPase activating protein of Ras, p120GAP, and regulates epidermal growth factor receptor-mediated signal transduction"*
**作者**：Theobald J, Hanby A, Patel K
**摘要**：研究发现Annexin VI抗体可阻断其与p120GAP的相互作用，揭示其通过调控Ras信号通路影响EGFR下游的细胞增殖与分化过程。
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若需更多文献或具体领域研究，可进一步补充关键词(如疾病模型或实验技术)。
**Background of Annexin VI Antibody**
Annexin VI is a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins involved in membrane trafficking, signal transduction, and cellular regulation. Annexin VI, encoded by the *ANXA6* gene, is unique among annexins due to its eight-repeat structure (most annexins have four), which likely contributes to its diverse functional roles. It is implicated in endocytosis, exocytosis, calcium signaling, and membrane repair, and has been associated with pathologies such as cancer, cardiovascular diseases, and diabetes.
Annexin VI antibodies are essential tools for studying the expression, localization, and function of this protein. These antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant Annexin VI proteins. They enable detection of Annexin VI in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Specificity is validated through knockout controls or siRNA knockdowns.
Research using Annexin VI antibodies has revealed its dual role in cancer progression, acting as both a tumor suppressor and promoter depending on cellular context. In cardiovascular studies, its involvement in membrane repair mechanisms highlights potential therapeutic targets. Commercial Annexin VI antibodies vary in clonality (monoclonal/polyclonal) and conjugation, catering to diverse experimental needs. Proper validation remains critical to ensure reliable results in studying Annexin VI's complex biological roles.
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