| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NL2; ARP4; FIAF; HARP; PGAR; HFARP; TGQTL; UNQ171; pp1158 |
| Entrez GeneID | 51129 |
| WB Predicted band size | Calculated MW: 45 kDa; Observed MW: 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human Angiopoietin-like 4 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Angiopoietin-like 4(ANGPTL4)抗体的文献示例(注：文献为虚构，仅用于演示格式)：
1. **"Monoclonal antibody targeting ANGPTL4 suppresses tumor metastasis in murine models"**
- **作者**: Li, X., Zhang, Y., & Wang, H.
- **摘要**: 该研究开发了一种针对ANGPTL4蛋白C端表位的单克隆抗体，通过抑制ANGPTL4与整合素的相互作用，显著减少小鼠模型中乳腺癌细胞的肺转移，提示其治疗潜力。
2. **"ANGPTL4 antibody-based ELISA detects elevated levels in obesity-associated metabolic syndrome"**
- **作者**: Chen, L., et al.
- **摘要**: 研究者验证了一种高灵敏度ELISA检测方法，使用兔源多克隆抗体定量人血清ANGPTL4水平，发现代谢综合征患者中ANGPTL4浓度与胰岛素抵抗呈正相关。
3. **"Neutralizing ANGPTL4 antibody ameliorates diabetic retinopathy by regulating vascular permeability"**
- **作者**: Kim, S., et al.
- **摘要**: 通过阻断ANGPTL4与VE-cadherin的结合，该中和抗体显著降低糖尿病小鼠视网膜血管渗漏，为治疗血管通透性相关疾病提供新策略。
(提示：实际文献需通过PubMed/Google Scholar检索关键词如“ANGPTL4 antibody application”或“ANGPTL4 immunoassay”获取。)
Angiopoietin-like 4 (ANGPTL4), a secretory glycoprotein belonging to the angiopoietin-like family, plays multifaceted roles in metabolic regulation, angiogenesis, and cancer progression. It is widely expressed in tissues, including liver, adipose, and endothelial cells, and is regulated by hypoxia, fasting, and inflammatory signals. ANGPTL4 functions as a lipoprotein lipase (LPL) inhibitor, modulating triglyceride metabolism and lipid partitioning. In cancer, it exhibits context-dependent effects—promoting tumor metastasis by enhancing vascular permeability and cell motility, while also suppressing angiogenesis in certain microenvironments.
Antibodies targeting ANGPTL4 are critical tools for studying its expression, localization, and functional mechanisms. They are employed in techniques like Western blotting, immunohistochemistry (IHC), and ELISA to quantify protein levels in physiological and pathological conditions, including obesity, diabetes, and cancer. Research using these antibodies has revealed conflicting roles of ANGPTL4 in diseases, potentially due to its tissue-specific isoforms or post-translational modifications. Recent studies also explore its therapeutic potential, with neutralizing antibodies being investigated for metabolic disorders or as adjuvants in cancer treatment. However, challenges remain in standardizing antibody specificity due to ANGPTL4’s structural complexity and cross-reactivity with family members.
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