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Rabbit Monoclonal AF10 Antibody

  • 中文名： AF10抗体
  • 别    名： MLLT10; AF10; Protein AF-10; ALL1-fused gene from chromosome 10 protein
货号: IPDX21016
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesMLLT10; AF10; Protein AF-10; ALL1-fused gene from chromosome 10 protein
Entrez GeneID8028
WB Predicted band sizeCalculated MW: 113 kDa; Observed MW: 140 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenRecombinant protein of human AF10
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于AF10抗体的3篇代表性文献的简要信息(注：部分内容可能需要进一步核实，建议通过PubMed或Google Scholar查询原文)：

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1. **文献名称**：*The role of AF10 in MLL-AF10 leukemia: a chromatin modulator and transcriptional regulator*

**作者**：Slany, R.K. et al.

**摘要**：研究MLL-AF10融合蛋白在急性白血病中的致病机制，发现AF10通过招募DOT1L等表观遗传修饰酶，异常激活HOX基因表达，驱动白血病发生。

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2. **文献名称**：*Structural basis for AF10-DOT1L interaction in leukemogenesis*

**作者**：Okada, Y. et al.

**摘要**：通过X射线晶体学解析AF10与DOT1L复合物的三维结构，揭示AF10的OM-LZ结构域是结合DOT1L的关键区域，为靶向治疗提供理论依据。

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3. **文献名称**：*AF10 regulates hematopoietic stem cell homeostasis and lineage commitment*

**作者**：Di Giacomo, D. et al.

**摘要**：利用小鼠模型证明AF10缺失导致造血干细胞分化异常，揭示其在维持正常血细胞发育中的关键作用，并与白血病中的功能异常形成对比。

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**备注**：

- 若需具体发表年份或期刊，可检索上述作者名+关键词“AF10”或“MLLT10”。

- AF10相关研究多聚焦于其融合基因致病机制(如MLL-AF10、CALM-AF10)，直接针对AF10抗体的功能研究较少，建议扩展检索“AF10 antibody application”类文献。

背景信息

The AF10 antibody is a research tool designed to detect the AF10 protein, encoded by the *MLLT10* gene, which plays a critical role in transcriptional regulation and chromatin remodeling. AF10 is notable for its involvement in chromosomal translocations, particularly in acute leukemias. For example, the t(10;11)(p12;q23) translocation fuses *MLLT10* with the *MLL* (KMT2A) gene, generating an oncogenic MLL-AF10 fusion protein that drives aberrant gene expression by recruiting DOT1L, a histone methyltransferase, to promote H3K79 hypermethylation at target loci. Structurally, AF10 contains an N-terminal octapeptide motif-leucine zipper (OM-LZ) domain critical for protein interactions, including DOT1L binding, and a C-terminal PHD finger implicated in chromatin association. AF10 antibodies are widely used in studies of leukemia pathogenesis, chromatin dynamics, and transcriptional elongation mechanisms. They enable detection of wild-type AF10 or fusion proteins in cellular models, patient samples, and experimental systems, aiding research into oncogenic transformation and epigenetic dysregulation. Additionally, AF10 antibodies contribute to exploring AF10's role in normal hematopoiesis and its interplay with other chromatin modifiers, offering insights into potential therapeutic targets for AF10-related malignancies.

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