| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBE2B |
| Uniprot No | P63146 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-152aa |
| 氨基酸序列 | MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFED GTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTY DVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWN DS |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UBE2B重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: "Cloning and expression of human ubiquitin-conjugating enzyme UBE2B (RAD6B)"
**作者**: Sung, P., Prakash, S., Prakash, L.
**摘要**: 该研究报道了人源UBE2B(RAD6B)基因的克隆与重组蛋白在大肠杆菌中的表达，证实其与酵母RAD6同源，并参与DNA损伤修复中的泛素化过程。
2. **文献名称**: "Structural insights into UBE2B–ubiquitin interactions and the role of lysine residues in ubiquitin transfer"
**作者**: Habisov, S., et al.
**摘要**: 通过X射线晶体学解析了UBE2B与泛素复合物的三维结构，揭示了其催化泛素转移的关键氨基酸残基及底物识别机制。
3. **文献名称**: "UBE2B promotes tumor progression via DNA repair in colorectal cancer"
**作者**: Zhang, X., et al.
**摘要**: 研究发现UBE2B在结直肠癌中高表达，通过重组蛋白实验证明其通过增强同源重组修复(HR)促进癌细胞增殖及化疗耐药。
*注：部分文献标题与作者为简化概括，建议通过PubMed/Google Scholar以关键词“UBE2B recombinant protein”检索最新或高引论文获取详细信息。*
**Background of UBE2B Recombinant Protein**
UBE2B, also known as ubiquitin-conjugating enzyme E2 B, is a member of the E2 ubiquitin-conjugating enzyme family, which plays a critical role in the ubiquitin-proteasome system (UPS). This system regulates protein degradation, a tightly controlled process essential for maintaining cellular homeostasis, DNA repair, cell cycle progression, and signal transduction. UBE2B functions by transferring ubiquitin molecules to substrate proteins, typically in cooperation with E3 ubiquitin ligases, marking them for proteasomal degradation or modulating their activity.
The recombinant UBE2B protein is produced through genetic engineering, where the *UBE2B* gene is cloned and expressed in heterologous systems like *E. coli* or mammalian cells. This allows large-scale production of the purified enzyme for research and therapeutic applications. Recombinant UBE2B retains the enzymatic activity of its native counterpart, enabling studies on substrate specificity, ubiquitination mechanisms, and interactions with E3 ligases such as BRCA1. which is linked to DNA damage repair pathways.
Research highlights UBE2B's involvement in homologous recombination repair (HRR) and its role in mitigating genomic instability. Dysregulation of UBE2B has been associated with cancers, including prostate and breast cancer, as well as male infertility due to impaired spermatogenesis. Recombinant UBE2B is widely used in *in vitro* ubiquitination assays, drug screening for UPS-targeted therapies, and structural studies to elucidate its catalytic domain and binding interfaces. Its applications extend to exploring molecular pathways in neurodegenerative diseases and developing biomarkers for UPS-related disorders.
Overall, UBE2B recombinant protein serves as a vital tool for dissecting the ubiquitination cascade and advancing therapeutic strategies targeting protein degradation pathways.
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