| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBA3 |
| Uniprot No | Q8TBC4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-463aa |
| 氨基酸序列 | MADGEEPEKKRRRIEELLAEKMAVDGGCGDTGDWEGRWNHVKKFLERSGP FTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVID MDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQD FNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLID GGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCI EYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYR LTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDG LYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSP AITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVT TPQTVLFKLHFTS |
| 预测分子量 | 73 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UBA3重组蛋白的3篇参考文献，按研究方向和内容分类整理：
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### 1. **Structural Basis of NEDD8 Activation by the APPBP1-UBA3 Heterodimer**
**作者**: Walden, H. et al.
**摘要**: 本研究解析了APPBP1-UBA3异源二聚体与NEDD8的晶体结构，揭示了UBA3在NEDD8激活过程中的催化机制。通过重组蛋白表达和纯化，证明了UBA3的腺苷酸化活性及与NEDD8的特异性结合，为NEDDylation通路的分子机制提供了结构基础。
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### 2. **Development of a High-Throughput Assay for NEDD8-Activating Enzyme Using Recombinant UBA3**
**作者**: Soucy, T.A. et al.
**摘要**: 研究者利用重组UBA3蛋白建立了一种高通量酶活性检测方法，用于筛选NEDD8激活酶(NAE)抑制剂。实验表明，重组UBA3在体外的腺苷酸化活性可定量表征，为癌症治疗中NAE靶向药物的开发提供了技术平台。
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### 3. **Recombinant UBA3 Protein Expression and Functional Characterization in E. coli**
**作者**: Huang, D.T. et al.
**摘要**: 该研究报道了在大肠杆菌中高效表达并纯化功能性重组UBA3蛋白的方法，通过体外酶活实验验证了其与APPBP1形成复合物的能力，并证实其对NEDD8的激活作用依赖于ATP水解，为后续生化研究提供了可靠的重组蛋白来源。
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**注**：以上文献为示例性质，实际引用需根据具体数据库(如PubMed、ScienceDirect)检索并核对原文信息。
**Background of UBA3 Recombinant Protein**
UBA3 (Ubiquitin-like modifier-activating enzyme 3) is a critical component of the NEDD8-activating enzyme (NAE) complex, which plays a central role in the post-translational modification pathway known as neddylation. This pathway involves the covalent attachment of the ubiquitin-like protein NEDD8 (neural precursor cell-expressed developmentally downregulated protein 8) to specific substrate proteins, primarily cullin family members within Cullin-RING ligases (CRLs). Neddylation regulates CRL activity, which is essential for the ubiquitination and subsequent proteasomal degradation of various cellular proteins, including those involved in cell cycle progression, DNA repair, and signal transduction.
UBA3 functions as the catalytic subunit of the NAE heterodimer (composed of UBA3 and APPBP1), where it activates NEDD8 via ATP-dependent adenylation and facilitates its transfer to target proteins. Dysregulation of UBA3 or the neddylation pathway has been implicated in numerous diseases, particularly cancers, where hyperactivation promotes tumor growth and resistance to therapy. Consequently, UBA3 has emerged as a potential therapeutic target, driving interest in recombinant UBA3 protein for mechanistic and drug discovery studies.
Recombinant UBA3 is typically produced in *E. coli* or mammalian expression systems, ensuring high purity and enzymatic activity. It is widely used in *in vitro* assays to study neddylation dynamics, screen for NAE inhibitors (e.g., MLN4924/pevonedistat), and explore interactions within the CRL regulatory network. Structural studies leveraging recombinant UBA3 have also elucidated its ATP-binding pocket and conformational changes during catalysis, providing insights for inhibitor design.
Research on UBA3 continues to advance our understanding of its role in cellular homeostasis and disease, positioning it as a key molecule in both basic biology and translational oncology.
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