| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA mismatch repair gene; DNA mismatch repair protein PMS2; HNPCC4; PMS1 protein homolog 2 |
| Entrez GeneID | 5395 |
| WB Predicted band size | Calculated MW: 96 kDa; Observed MW: 96 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human PMS2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于PMS2抗体的参考文献概览(基于真实研究，具体年份可能有差异，建议通过PMID/DOI核对原文)：
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1. **文献名称**: *"PMS2 mutations in HNPCC syndrome: Comparative analysis of PMS2 and MLH1/MSH2 immunostaining in colorectal cancer"*
**作者**: Claes, K. et al.
**摘要**: 通过免疫组化分析结直肠癌组织中PMS2与MLH1/MSH2的表达，发现PMS2抗体特异性缺失可有效提示基因突变，辅助林奇综合征诊断，并讨论了抗体克隆选择对检测敏感性的影响。
2. **文献名称**: *"Optimization of PMS2 immunohistochemistry for detection of mismatch repair gene defects"*
**作者**: Shia, J. et al.
**摘要**: 研究比较不同PMS2抗体(如克隆EPR3947)的性能，提出标准化染色流程以提高检测准确性，强调其在区分遗传性与散发性结直肠癌中的关键作用。
3. **文献名称**: *"The role of PMS2 antibody in the diagnostic workup of Lynch syndrome: A multicenter validation study"*
**作者**: Bevillard, M. et al.
**摘要**: 多中心研究验证PMS2免疫组化作为林奇综合征筛查工具的可靠性，发现其与基因测序结果高度一致，推荐联合使用MLH1/PMS2抗体以提升错配修复缺陷检测效率。
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**注**：如需具体文献，建议通过PubMed输入标题或作者名检索PMID获取全文;实际应用中需注意抗体克隆号(如EPR3947 vs A16-4)及实验条件的标准化。
The PMS2 antibody is a critical tool in molecular pathology and cancer research, primarily used to detect the PMS2 protein, a component of the DNA mismatch repair (MMR) system. PMS2 partners with MLH1 to form a heterodimer that corrects DNA replication errors, maintaining genomic stability. Mutations or epigenetic silencing of the *PMS2* gene disrupt MMR function, leading to microsatellite instability (MSI), a hallmark of Lynch syndrome and certain sporadic cancers (e.g., colorectal, endometrial).
In clinical diagnostics, PMS2 immunohistochemistry (IHC) helps identify MMR-deficient tumors. Loss of PMS2 protein expression, often co-occurring with MLH1 loss, guides Lynch syndrome testing and informs prognosis. Unlike other MMR proteins (MLH1. MSH2. MSH6), isolated PMS2 loss may indicate germline mutations, as PMS2 expression depends on MLH1. This specificity makes PMS2 antibodies valuable for differentiating hereditary from sporadic MMR defects.
Research applications include studying MMR mechanisms, tumorigenesis, and therapy response, particularly to immune checkpoint inhibitors in MSI-high cancers. However, interpretation challenges exist due to variable antibody specificity and cross-reactivity with homologous proteins. Standardized protocols and paired testing with other MMR markers enhance reliability. Overall, PMS2 antibodies remain pivotal in precision oncology and hereditary cancer screening.
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