| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TXNL4B |
| Uniprot No | Q9NX01 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-149aa |
| 氨基酸序列 | MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMSFLLPKLTSKKEV DQAIKSTAEKVLVLRFGRDEDPVCLQLDDILSKTSSDLSKMAAIYLVDVD QTAVYTQYFDISYIPSTVFFFNGQHMKVDYGSPDHTKFVGSFKTKQDFID LIEVIYRGAMRGKLIVQSPIDPKNIPKYDLLYQDI |
| 预测分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TXNL4B重组蛋白的虚构参考文献示例(实际文献需通过学术数据库查询)：
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1. **文献名称**：*Recombinant TXNL4B Protein Attenuates Oxidative Stress in Neurodegenerative Cell Models*
**作者**：Chen L, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组TXNL4B蛋白，验证其硫氧还蛋白活性。实验表明，重组TXNL4B可减少神经细胞模型中活性氧(ROS)积累，并抑制淀粉样蛋白诱导的凋亡，提示其在神经退行性疾病中的潜在治疗价值。
2. **文献名称**：*Structural Insights into TXNL4B Interaction with p53: Implications for Cancer Therapy*
**作者**：Wang Y, et al.
**摘要**：通过X射线晶体学解析了重组TXNL4B蛋白的三维结构，并发现其通过二硫键异构酶活性调控p53蛋白的稳定性。重组TXNL4B的过表达增强了p53依赖的肿瘤细胞凋亡，为癌症靶向治疗提供了新思路。
3. **文献名称**：*Optimized Expression and Functional Characterization of Recombinant TXNL4B in Mammalian Cells*
**作者**：Kim S, et al.
**摘要**：本文开发了一种基于哺乳动物细胞(HEK293)的重组TXNL4B高效表达系统，纯化蛋白显示出更高的催化活性。功能实验表明，重组TXNL4B通过调节NF-κB信号通路抑制炎症反应。
4. **文献名称**：*TXNL4B Recombinant Protein Enhances Chemosensitivity in Colorectal Cancer*
**作者**：Garcia-Ruiz C, et al.
**摘要**：研究证实重组TXNL4B蛋白可逆转结直肠癌细胞对5-FU的耐药性。机制研究表明，其通过硫氧还蛋白系统恢复氧化还原平衡，从而增强化疗药物诱导的DNA损伤反应。
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**注意**：以上为模拟示例，实际文献需通过PubMed、Web of Science或Google Scholar搜索**TXNL4B recombinant protein**或**TXNL4B function**等关键词获取。建议结合具体研究方向筛选近年高影响力论文。
**Background of TXNL4B Recombinant Protein**
TXNl4B (Thioredoxin-like 4B) is a member of the thioredoxin (Trx) family, a group of redox-active proteins critical for maintaining cellular redox homeostasis. This protein contains a conserved Trx-fold domain with a CXXC motif, enabling its participation in redox reactions by facilitating disulfide bond reduction. TXNL4B is primarily localized in the nucleus and cytoplasm, where it interacts with components of the mRNA splicing machinery, suggesting roles in post-transcriptional gene regulation.
Recombinant TXNL4B is engineered via molecular cloning, expressing the protein in systems like *E. coli* or mammalian cells to ensure proper folding and activity. Its recombinant form retains enzymatic activity, making it valuable for studying redox-dependent processes, protein-protein interactions, and spliceosome dynamics. Research highlights its involvement in cellular stress responses, DNA repair, and apoptosis, with dysregulation linked to cancer progression, neurodegenerative disorders, and metabolic diseases.
In drug discovery, recombinant TXNL4B serves as a tool for screening inhibitors targeting redox pathways or splicing mechanisms. It is also used to explore post-translational modifications (e.g., phosphorylation) that regulate its function. High-purity recombinant TXNL4B, often tagged with His or GST for purification, supports structural studies (e.g., crystallography) to map interaction sites or catalytic residues.
Overall, TXNL4B recombinant protein bridges molecular biology and translational research, offering insights into redox biology, RNA processing, and disease mechanisms.
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