| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TXNDC12 |
| Uniprot No | O95881 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-172aa |
| 氨基酸序列 | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMHN GLGKGFGDHI HWRTLEDGKK EAAASGLPLM VIIHKSWCGA CKALKPKFAE STEISELSHN FVMVNLEDEE EPKDEDFSPD GGYIPRILFL DPSGKVHPEI INENGNPSYK YFYVSAEQVV QGMKEAQERL TGDAFRKKHL EDEL |
| 预测分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TXNDC12重组蛋白的3篇示例文献(内容为虚构示例，仅作参考)：
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1. **文献名称**: *Recombinant Expression and Functional Characterization of Human TXNDC12 in E. coli*
**作者**: Smith J, et al.
**摘要**: 研究报道了在大肠杆菌中高效表达并纯化人源TXNDC12重组蛋白的方法，证实其具有硫氧还蛋白活性，并可通过调控内质网应激通路减少氧化损伤。
2. **文献名称**: *Structural Insights into TXNDC12’s Role in Protein Disulfide Isomerization*
**作者**: Lee S, et al.
**摘要**: 通过X射线晶体学解析TXNDC12重组蛋白的三维结构，揭示了其独特的二硫键异构酶活性位点，并证明其参与错误折叠蛋白的修复过程。
3. **文献名称**: *TXNDC12 Recombinant Protein Attenuates Neuroinflammation in Parkinson’s Disease Models*
**作者**: Zhang Y, et al.
**摘要**: 利用重组TXNDC12蛋白进行体外和动物实验，发现其通过抑制NLRP3炎症小体活化，显著减轻帕金森病模型的神经炎症和神经元凋亡。
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*注：以上文献信息为模拟示例，实际文献需通过PubMed、Web of Science等数据库检索确认。*
**Background of TXNDC12 Recombinant Protein**
TXNDC12 (Thioredoxin Domain-Containing Protein 12), also known as Endoplasmic Reticulum Resident Thioredoxin Superfamily Member 12 (ERTR12), is a member of the thioredoxin protein family characterized by a conserved thioredoxin-like domain. This protein is primarily localized in the endoplasmic reticulum (ER), where it plays a critical role in maintaining redox homeostasis and facilitating protein folding through its disulfide isomerase activity. TXNDC12 is involved in resolving misfolded proteins by catalyzing the rearrangement of disulfide bonds, a process essential for proper protein maturation and ER stress mitigation.
Dysregulation of TXNDC12 has been linked to various pathological conditions, including cancer, neurodegenerative diseases, and metabolic disorders. For instance, its overexpression has been observed in certain tumors, where it may promote cell survival under hypoxic or oxidative stress. Conversely, reduced TXNDC12 activity is associated with ER stress accumulation, contributing to neurodegenerative processes like Parkinson’s disease.
Recombinant TXNDC12 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce high-purity, functional protein for research and therapeutic applications. This recombinant form retains the enzymatic activity of native TXNDC12. enabling studies on redox regulation, protein-protein interactions, and ER stress pathways. It is widely utilized in *in vitro* assays, cell-based models, and drug discovery platforms targeting diseases linked to protein misfolding or oxidative damage.
The development of recombinant TXNDC12 has advanced understanding of its structural and functional roles, offering potential avenues for therapeutic interventions aimed at modulating ER stress responses or enhancing cellular resilience in pathological states.
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