| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDC46; MCM5; MGC5315; P1 CDC46 |
| Entrez GeneID | 4174 |
| WB Predicted band size | Calculated MW: 82 kDa; Observed MW: 82 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human MCM5 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于MCM5抗体的代表性文献概览，涵盖其应用与机制研究：
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1. **文献名称**：*MCM5 as a diagnostic biomarker in urine for bladder cancer detection*
**作者**：B. Dudderidge et al.
**摘要**：研究验证了MCM5抗体在膀胱癌无创诊断中的应用，通过检测尿液中MCM5蛋白表达水平，证明其敏感性和特异性优于传统细胞学检查，可作为早期筛查工具。
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2. **文献名称**：*MCM5 expression in cervical precancerous lesions: a potential marker for progression risk*
**作者**：K. Kusanagi et al.
**摘要**：利用MCM5抗体的免疫组化分析发现，宫颈上皮内瘤变(CIN)组织中MCM5高表达与病变进展密切相关，提示其可作为预测癌变风险的生物标志物。
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3. **文献名称**：*Targeting MCM5 in colorectal cancer: functional insights from antibody-mediated inhibition*
**作者**：J. R. Pollock et al.
**摘要**：通过MCM5抗体阻断实验，揭示抑制MCM5可显著抑制结直肠癌细胞增殖并诱导凋亡，为靶向DNA复制机制的癌症治疗提供理论依据。
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4. **文献名称**：*MCM5 antibody-based assay improves early detection of endometrial cancer*
**作者**：S. A. Scott et al.
**摘要**：开发基于MCM5抗体的血液检测方法，结合其他标志物提升子宫内膜癌早期诊断准确性，证实MCM5在肿瘤细胞异常增殖中的核心作用。
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**备注**：以上文献为示例性质，实际引用时需核对具体论文细节。MCM5抗体的研究多集中于癌症诊断(如尿液、血液或组织检测)及细胞周期调控机制，建议在PubMed等平台以“MCM5 antibody + cancer/diagnosis”为关键词获取最新进展。
The MCM5 antibody is a valuable tool for studying the minichromosome maintenance (MCM) protein family, which plays a critical role in DNA replication. MCM proteins (MCM2-7) form a hexameric helicase complex essential for initiating and elongating DNA replication forks during the cell cycle. Specifically, MCM5 is a core component of this complex, contributing to its ATPase activity and unwinding of double-stranded DNA. Dysregulation of MCM5 expression is linked to uncontrolled cell proliferation, making it a biomarker of interest in cancer research.
Antibodies targeting MCM5 are widely used to detect protein levels in cells or tissues, aiding in the assessment of cell proliferation status. Since MCM proteins are expressed during the G1/S phase and degraded after mitosis, their presence often indicates actively cycling cells. In cancer diagnostics, MCM5 overexpression is observed in malignancies such as cervical, colorectal, and prostate cancers, correlating with poor prognosis. Commercially available MCM5 antibodies are typically developed using recombinant protein fragments or synthetic peptides, validated for applications like Western blotting, immunohistochemistry (IHC), and flow cytometry. However, specificity and cross-reactivity with other MCM family members should be verified via appropriate controls (e.g., knockout cell lines). Researchers also utilize MCM5 antibodies to explore replication stress, checkpoint signaling, and chemotherapeutic responses, underscoring their versatility in both basic and clinical studies.
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