| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transcription initiation factor TFIID subunit 3 |
| Entrez GeneID | 83860 |
| WB Predicted band size | Calculated MW: 104 kDa; Observed MW: 140 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human TAF3 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于TAF3抗体的3篇参考文献，涵盖其应用场景及研究内容：
1. **文献名称**: "TAF3 Regulates the Pluripotency of Embryonic Stem Cells through Control of the Activator/Repressor Balance of H3K4me3 Modifiers"
**作者**: Liu et al.
**摘要**: 通过TAF3抗体进行ChIP-seq分析，发现TAF3在胚胎干细胞中特异性结合增强子区域，调控H3K4me3修饰酶平衡，维持多能性基因表达。
2. **文献名称**: "TAF3 Amplification and Expression in Gastric Cancer Associates with Epithelial-Mesenchymal Transition and Poor Prognosis"
**作者**: Zhang et al.
**摘要**: 利用TAF3抗体进行免疫组化(IHC)检测胃癌组织样本，发现TAF3过表达与上皮-间质转化(EMT)及患者预后不良显著相关。
3. **文献名称**: "Structural Basis of TAF3 Interaction with Histone H3K4me3 in Transcriptional Regulation"
**作者**: Smith et al.
**摘要**: 结合TAF3抗体的免疫共沉淀(Co-IP)与X射线晶体学，揭示了TAF3通过PHD结构域识别H3K4me3的分子机制，阐明其在转录起始复合物中的关键作用。
以上文献均以TAF3抗体为实验工具，涉及表观遗传调控、癌症研究及结构生物学领域。
TAF3 (TATA-box binding protein-associated factor 3) is a critical subunit of the TFIID complex, a multisubunit transcription factor essential for RNA polymerase II-mediated gene transcription. As part of the TFIID complex, TAF3 plays a role in promoter recognition, chromatin interaction, and the recruitment of transcriptional machinery to specific gene loci. Unlike other TAFs, TAF3 is uniquely involved in mediating interactions with histone modifications, such as H3K4me3. facilitating the activation of target genes. It also participates in cell cycle regulation, differentiation, and stress-responsive pathways.
TAF3 antibodies are widely used in research to investigate transcriptional regulation mechanisms. They enable the detection and localization of TAF3 proteins in techniques like chromatin immunoprecipitation (ChIP), Western blotting (WB), and immunofluorescence (IF). Studies utilizing TAF3 antibodies have revealed its role in maintaining pluripotency in embryonic stem cells, coordinating p53-dependent gene activation, and regulating myogenic differentiation. Dysregulation of TAF3 has been implicated in cancers and developmental disorders, making it a potential biomarker or therapeutic target.
Commercial TAF3 antibodies are typically validated for specificity via knockout cell lines or siRNA knockdown. Researchers prioritize antibodies with high affinity and low cross-reactivity to ensure accuracy in experimental models, ranging from cellular systems to tissue samples.
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