| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Gnb2; Gnb2l1; RACK1; Transducin beta chain 2 |
| Entrez GeneID | 2783 |
| WB Predicted band size | Calculated MW: 37 kDa; Observed MW: 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human GNB2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于GNB2抗体的3篇模拟参考文献示例(内容基于公开研究领域知识整理，非真实文献)：
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1. **文献名称**: *GNB2 Antibody Validation in Neurodevelopmental Disorder Models*
**作者**: Smith J, et al.
**摘要**: 研究通过Western blot和免疫荧光验证了GNB2抗体在神经细胞模型中的特异性，发现GNB2蛋白表达水平在特定神经发育障碍小鼠模型中显著下调，提示其可能与突触信号传导异常相关。
2. **文献名称**: *Role of GNB2 in GPCR Signaling Pathways: Antibody-Based Profiling*
**作者**: Lee S, et al.
**摘要**: 文章利用GNB2抗体探究其在G蛋白偶联受体(GPCR)信号复合体中的定位，证实GNB2与Gγ亚基的相互作用对下游cAMP调控至关重要，并建立了抗体在HEK293细胞系中的功能性应用标准。
3. **文献名称**: *GNB2 Overexpression in Cancer: Immunohistochemical Analysis*
**作者**: Wang R, et al.
**摘要**: 研究采用GNB2抗体对多种癌症组织进行免疫组化分析，发现乳腺癌和肺癌中GNB2蛋白高表达与患者预后不良相关，提示其作为潜在生物标志物的可能性。
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注：以上文献为模拟示例，实际引用需通过PubMed或Google Scholar等平台检索真实文献。
The GNB2 antibody targets the beta-2 subunit of heterotrimeric G proteins, which are critical components of intracellular signaling pathways. G proteins, composed of α, β, and γ subunits, mediate signal transduction from G protein-coupled receptors (GPCRs) to downstream effectors, regulating processes like cell proliferation, differentiation, and gene expression. The GNB2 subunit, encoded by the GNB2 gene, stabilizes the Gβγ complex and participates in interactions with GPCRs, effectors (e.g., adenylyl cyclase, ion channels), and regulatory proteins. Dysregulation of GNB2 has been linked to neurological disorders, cancers, and cardiovascular diseases, making it a focus in pathophysiological research.
GNB2 antibodies are essential tools for studying the expression, localization, and function of the Gβ2 subunit. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to assess protein levels in tissues or cell lines. These antibodies aid in exploring GNB2's role in disease mechanisms, such as its potential involvement in tumor progression via aberrant signaling pathways or its impact on neural function. Commercial GNB2 antibodies are typically developed in hosts like rabbits or mice, with validation for specificity and cross-reactivity across species. Researchers rely on these reagents to dissect G protein-mediated signaling cascades and evaluate therapeutic targets in diseases associated with GPCR dysfunction.
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