| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IMPD; RP10; IMPD1; LCA11; sWSS2608 |
| WB Predicted band size | 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human IMPDH1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于Tryptophanyl-tRNA Synthetase(WARS)抗体的文献摘要(注：实际文献需根据具体研究需求检索，以下为模拟示例)：
1. **文献名称**：*"Autoantibodies to Tryptophanyl-tRNA Synthetase in Dermatomyositis Patients"*
**作者**：Smith J, et al.
**摘要**：该研究报道了在皮肌炎患者血清中发现针对色氨酸-tRNA合成酶(WARS)的自身抗体，并通过ELISA和Western blot验证了抗体的特异性，提示WARS可能参与自身免疫疾病的分子机制。
2. **文献名称**：*"Structural Characterization of Tryptophanyl-tRNA Synthetase Using Monoclonal Antibodies"*
**作者**：Lee H, et al.
**摘要**：通过制备针对人源WARS的单克隆抗体，解析了其抗原表位并用于酶活性抑制实验，揭示了WARS催化结构域的功能区域，为开发靶向抑制剂提供了工具。
3. **文献名称**：*"Upregulation of Tryptophanyl-tRNA Synthetase as a Biomarker in Colorectal Cancer"*
**作者**：Wang Y, et al.
**摘要**：研究利用免疫组化(IHC)和抗WARS抗体发现结直肠癌组织中WARS表达显著升高，提示其可能作为癌症预后标志物，并探讨了其在肿瘤代谢中的作用。
4. **文献名称**：*"Cross-Species Reactivity of Anti-Tryptophanyl-tRNA Synthetase Antibodies in Disease Models"*
**作者**：Garcia R, et al.
**摘要**：评估了多种物种(人、小鼠、大鼠)中WARS抗体的交叉反应性，验证了其在动物疾病模型中的适用性，为临床前研究提供抗体选择依据。
(注：以上文献信息为示例性虚构，实际文献需通过PubMed或Google Scholar检索关键词“Tryptophanyl-tRNA Synthetase antibody”获取。)
Tryptophan tRNA ligase, commonly known as Tryptophanyl-tRNA synthetase (TrpRS or WARS), is a member of the aminoacyl-tRNA synthetase (aaRS) family. This enzyme catalyzes the attachment of tryptophan to its cognate tRNA during protein translation, ensuring the accurate incorporation of tryptophan into nascent polypeptide chains. Beyond its canonical role in translation, TrpRS has been implicated in non-canonical functions, including immune signaling, angiogenesis regulation, and cellular stress responses. For instance, alternative splicing or post-translational modifications can produce truncated isoforms of TrpRS that exhibit cytokine-like activity, influencing pathways such as the IFN-γ signaling network.
Antibodies targeting TrpRS are critical tools for studying its expression, localization, and function in both physiological and pathological contexts. Researchers employ these antibodies in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate TrpRS dynamics in cellular models, tissues, or disease states. Dysregulation of TrpRS has been linked to cancers, neurodegenerative disorders, and autoimmune diseases, making such antibodies valuable for biomarker discovery or therapeutic research. Specificity validation (e.g., knockout controls) is essential due to potential cross-reactivity with other aaRS family members. Additionally, species reactivity (human, mouse, rat) should be confirmed for experimental relevance. These antibodies also facilitate studies exploring TrpRS's dual roles in translation fidelity and extracellular signaling, bridging molecular biology and clinical research.
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