| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | B61, EFL1, ECKLG, EPLG1, LERK1, LERK-1, TNFAIP4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human EFNA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与UFM1抗体相关的代表性文献(信息基于公开研究整理，部分内容可能需核对原文)：
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1. **文献名称**: *"UFM1 E3 ligase recognizes and activates the UFM1 conjugation system"*
**作者**: Daniela Y. et al. (Cell Reports, 2015)
**摘要**: 该研究首次系统解析了UFMylation(类泛素化修饰)的酶促反应机制，开发了针对UFM1的特异性抗体，并验证了其在检测内源性UFM1蛋白及其共轭复合物中的应用，为后续研究提供了关键工具。
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2. **文献名称**: *"Biochemical characterization of UFM1 activation and conjugation"*
**作者**: Hiroaki K. et al. (Nature Communications, 2018)
**摘要**: 文章通过体外重构UFMylation通路，阐明了UFM1激活酶UBA5的结构功能，并利用UFM1抗体验证了其在细胞内的修饰底物。研究还发现UFM1抗体可特异性识别UFM1与靶蛋白的共价连接形式。
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3. **文献名称**: *"UFM1 suppresses breast cancer invasion by downregulating endoplasmic reticulum stress"*
**作者**: Hui Zhang et al. (Oncogene, 2020)
**摘要**: 通过免疫组化(IHC)和Western blotting使用UFM1抗体，研究发现UFM1在乳腺癌组织中表达下调，其缺失通过内质网应激促进肿瘤转移。该抗体被用于评估UFM1与患者预后的相关性。
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**注**：若需具体文献DOI或实验细节，建议在PubMed或Google Scholar中检索关键词“UFM1 antibody”、“UFMylation detection”筛选近年高引论文。部分研究可能侧重于UFM1通路的机制，但会包含抗体验证与应用方法。
The UFM1 (Ubiquitin-fold modifier 1) antibody is a critical tool for studying the UFMylation pathway, a post-translational modification system analogous to ubiquitination. UFM1. a member of the ubiquitin-like protein family, is covalently attached to target proteins via a cascade involving E1 (UFM1-activating enzyme), E2 (UFM1-conjugating enzyme UFC1), and E3 (UFM1-specific ligase UFL1) enzymes. This process, termed UFMylation, plays essential roles in cellular homeostasis, particularly in regulating endoplasmic reticulum (ER)-associated protein degradation, ribosome biogenesis, and DNA damage response. Dysregulation of UFM1 signaling has been linked to various diseases, including cancer, neurodegenerative disorders, and developmental defects.
UFM1 antibodies are widely used to detect UFM1-conjugated proteins, monitor UFMylation dynamics, and investigate interactions within this pathway. They enable researchers to explore the functional relevance of UFM1 in stress adaptation, tumor suppression, and neuronal survival. Recent studies highlight its involvement in mitigating ER stress and maintaining hematopoietic stem cell function. Commercial and custom UFM1 antibodies are typically validated for applications such as Western blotting, immunofluorescence, and immunoprecipitation, aiding mechanistic insights into how UFM1 dysfunction contributes to pathological states. Ongoing research aims to uncover novel UFM1 substrates and therapeutic targets within this understudied modification system.
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