| WB | 咨询技术 | Human,Monkey |
| IF | 咨询技术 | Human,Monkey |
| IHC | 1/50-1/100 | Human,Monkey |
| ICC | 1/50-1/200 | Human,Monkey |
| FCM | 咨询技术 | Human,Monkey |
| Elisa | 咨询技术 | Human,Monkey |
| Aliases | FMR1; Fragile X mental retardation protein 1; FMRP; Protein FMR-1 |
| Entrez GeneID | 2332 |
| WB Predicted band size | Calculated MW: 71 kDa; Observed MW: 70-80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Monkey |
| Immunogen | A synthetic peptide of human FMRP |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于FMRP抗体的3-4篇参考文献及其摘要：
1. **"Antibody characterization for the detection of FMRP"**
- **作者**: Iwahashi, C., Tassone, F., Hagerman, R.J., et al.
- **摘要**: 该研究比较了多种市售FMRP抗体的特异性，评估其在人脑组织及血液样本中的检测效果，强调了抗体选择对实验可靠性的重要性。
2. **"FMR1 protein: conserved expression and axonal targeting in fragile X syndrome"**
- **作者**: Devys, D., Lutz, Y., Rouyer, N., et al.
- **摘要**: 首次利用FMRP抗体定位蛋白在神经元的分布，发现脆性X综合征患者中FMRP表达显著缺失，揭示了其与认知障碍的直接关联。
3. **"FMRP as a potential biomarker for autism spectrum disorders"**
- **作者**: Wang, H., Doering, L.C.
- **摘要**: 研究通过FMRP抗体检测自闭症患者血液样本中的蛋白水平，提出FMRP可能作为神经发育障碍的生物学标志物。
4. **"FMRP detection in clinical diagnostics: a novel approach for fragile X syndrome screening"**
- **作者**: Tassone, F., Hagerman, R.J., Taylor, A.K., et al.
- **摘要**: 开发基于FMRP抗体的ELISA方法用于快速筛查脆性X综合征，验证其在临床诊断中的敏感性和特异性。
这些文献涵盖了抗体特异性验证、机制研究、生物标志物探索及临床应用等方向。
FMRP (Fragile X Mental Retardation Protein) antibodies are essential tools for studying the function and expression of FMRP, an RNA-binding protein encoded by the *FMR1* gene. FMRP plays a critical role in regulating synaptic plasticity and protein synthesis in neurons, particularly by transporting and stabilizing target mRNAs involved in neurodevelopment. Mutations in *FMR1*, such as CGG repeat expansions leading to gene silencing, result in the loss of FMRP and cause Fragile X syndrome (FXS), the most common inherited form of intellectual disability and a leading genetic cause of autism spectrum disorders.
FMRP antibodies are widely used in research to detect FMRP expression levels, cellular localization (e.g., cytoplasmic or synaptic compartments), and interactions with mRNA or other proteins. These antibodies are crucial for understanding FMRP’s role in neurodevelopment and its dysregulation in FXS. Clinically, FMRP antibodies aid in diagnostic assays (e.g., Western blot, immunohistochemistry) to confirm FXS in patients with *FMR1* mutations. They also support therapeutic research, such as evaluating FMRP restoration in experimental treatments. However, variability in antibody specificity (due to epitope differences or cross-reactivity) requires careful validation to ensure reliable results. Overall, FMRP antibodies remain pivotal in unraveling the molecular basis of FXS and advancing targeted therapies.
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