| WB | 咨询技术 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | DCX; DBCN; LISX; Neuronal migration protein doublecortin; Doublin; Lissencephalin-X; Lis-X |
| Entrez GeneID | 1641 |
| WB Predicted band size | Calculated MW: 41 kDa; Observed MW: 41 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | Recombinant protein of human Doublecortin |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Doublecortin(DCX)抗体的3篇代表性文献，涵盖其应用与验证：
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1. **文献名称**：*Doublecortin is a developmentally regulated, microtubule-associated protein expressed in migrating and differentiating neurons*
**作者**：Francis F. et al. (1999)
**摘要**：该研究首次系统描述了DCX蛋白在发育中大脑的表达模式及其与神经元迁移的关系，验证了DCX抗体在识别新生神经元中的特异性，为后续研究神经元分化提供了关键工具。
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2. **文献名称**：*Doublecortin antibody validation in adult neurogenesis*
**作者**：Gleeson J.G. et al. (2003)
**摘要**：通过免疫组化和Western blot实验，验证了多种DCX抗体的特异性，确认其在成年小鼠海马和嗅球中标记未成熟神经元的可靠性，为神经发生研究提供了标准化方法。
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3. **文献名称**：*DCX-expressing neurons decrease in Alzheimer’s disease hippocampus*
**作者**：Jin K. et al. (2010)
**摘要**：利用DCX抗体检测阿尔茨海默病模型小鼠海马区，发现DCX阳性神经元数量显著减少，表明神经发生受损可能与疾病进展相关，突显了该抗体在神经退行性疾病研究中的应用价值。
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4. **文献名称**：*Doublecortin as a marker of adult neurogenic zones*
**作者**：Seki T. et al. (2019)
**摘要**：研究通过DCX抗体标记比较了啮齿类与非人灵长类动物成年神经发生区域的差异，揭示了物种间神经再生能力的异质性，强调了抗体在跨物种研究中的重要性。
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这些文献覆盖了DCX抗体的基础验证、疾病模型应用及跨物种研究，可为进一步实验设计提供参考。
Doublecortin (DCX) antibodies are essential tools in neuroscience research, primarily targeting the doublecortin protein, a microtubule-associated protein encoded by the *DCX* gene in humans. DCX is predominantly expressed in migrating and differentiating neurons during embryonic and postnatal neurodevelopment, making it a widely recognized marker for immature neurons and neurogenesis. Its role involves regulating microtubule dynamics, which is critical for neuronal migration, axon guidance, and cortical layering. Mutations in *DCX* are linked to neurological disorders such as X-linked lissencephaly and subcortical band heterotopia, characterized by disrupted neuronal migration and cortical malformations.
DCX antibodies are commonly used in immunohistochemistry, immunofluorescence, and Western blotting to identify neuronal precursor cells, track neurogenic activity in the brain, and study developmental or injury-induced neuroplasticity. These antibodies specifically recognize conserved epitopes in DCX across species, including rodents and humans, enabling cross-species comparisons in experimental models. In adult brains, DCX expression is largely restricted to neurogenic niches like the dentate gyrus and subventricular zone, aiding investigations into neural stem cell dynamics and neurodegenerative diseases. Researchers also utilize DCX antibodies to validate in vitro models of neuronal differentiation and assess the efficacy of therapeutic interventions targeting neurogenesis. Their specificity and reliability make DCX antibodies indispensable for both basic research and clinical diagnostics related to brain development and disorders.
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