| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | minichromosome maintenance complex component 6; Mis5; P105MCM; MCG40308 |
| Entrez GeneID | 4175 |
| WB Predicted band size | Calculated MW: 93 kDa; Observed MW: 93 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human MCM6 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于MCM6抗体的3篇参考文献示例(注：以下内容为模拟示例，具体文献需根据实际数据库检索验证)：
1. **文献名称**: "MCM6 as a novel proliferation marker in human gliomas"
**作者**: Smith A, et al.
**摘要**: 本研究利用MCM6抗体通过免疫组化技术分析脑胶质瘤组织，发现MCM6高表达与肿瘤分级和预后不良显著相关，提示其可作为胶质瘤增殖活性的潜在生物标志物。
2. **文献名称**: "Validation of MCM6 antibody for detecting replication stress in colorectal cancer cells"
**作者**: Chen L, et al.
**摘要**: 通过Western blot和免疫荧光验证MCM6抗体的特异性，研究表明MCM6在结直肠癌细胞复制应激反应中表达上调，抗体可用于评估DNA复制异常相关的肿瘤进展。
3. **文献名称**: "MCM6 regulates lactase persistence by binding to the LCT promoter: Insights from antibody-based chromatin immunoprecipitation (ChIP)"
**作者**: Gonzalez JM, et al.
**摘要**: 使用MCM6抗体进行ChIP实验，发现MCM6通过与乳糖酶基因(LCT)启动子结合参与乳糖耐受的遗传调控，为乳糖酶持续性表型提供了分子机制证据。
如需具体文献，建议通过PubMed或Google Scholar以关键词“MCM6 antibody”、“MCM6 immunohistochemistry”或“MCM6 biomarker”检索近年研究。
The MCM6 antibody targets the minichromosome maintenance complex component 6 (MCM6), a critical protein involved in DNA replication. As part of the MCM2-7 hexameric complex, MCM6 functions as a DNA helicase, unwinding double-stranded DNA during replication initiation and elongation. This complex acts as a licensing factor, ensuring DNA is replicated only once per cell cycle. MCM6 is widely studied for its role in cell proliferation, making it a biomarker for cancer research. Overexpression of MCM6 correlates with aggressive tumor behavior and poor prognosis in various cancers, as it reflects uncontrolled cellular replication.
In addition to cancer, MCM6 is linked to lactase persistence. A single-nucleotide polymorphism (SNP) in the MCM6 gene (rs4988235) regulates lactase (LCT) expression, influencing lactose tolerance in adults. Antibodies against MCM6 are thus used in genetic and epigenetic studies of this trait.
MCM6 antibodies are essential tools in molecular biology, enabling detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Commercial antibodies are typically produced in hosts like rabbits or mice, using recombinant MCM6 protein fragments as immunogens. Researchers rely on these antibodies to explore DNA replication mechanisms, cell cycle regulation, and disease biomarkers, emphasizing their diagnostic and therapeutic potential.
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