| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ELOVL FA elongase 5; Fatty acid elongase 1; hELO1; Very long chain 3-ketoacyl-CoA synthase 5 |
| Entrez GeneID | 60481 |
| WB Predicted band size | Calculated MW: 35 kDa; Observed MW: 35 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human ELOVL5 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是关于ELOVL5抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*ELOVL5-mediated fatty acid elongation promotes cellular proliferation and invasion in hepatocellular carcinoma*
**作者**：Li, X., et al.
**摘要**：该研究利用ELOVL5特异性抗体进行免疫组化和Western blot分析，发现ELOVL5在肝癌组织中高表达，并通过调控脂肪酸延长过程促进肿瘤细胞增殖和转移。
2. **文献名称**：*Role of ELOVL5 in the biosynthesis of polyunsaturated fatty acids: Insights from knockout mouse models*
**作者**：Moon, Y.A., et al.
**摘要**：通过构建ELOVL5基因敲除小鼠，结合ELOVL5抗体检测蛋白表达，揭示了ELOVL5在肝脏中特异性催化ω-3和ω-6多不饱和脂肪酸合成的关键作用。
3. **文献名称**：*Antibody-based profiling of ELOVL isoforms in human adipose tissue: Implications for metabolic disorders*
**作者**：Wang, H., & Smith, S.R.
**摘要**：研究开发了一种针对ELOVL家族蛋白(包括ELOVL5)的高特异性抗体，用于人脂肪组织样本分析，发现ELOVL5表达水平与胰岛素抵抗呈负相关，提示其在脂代谢调控中的潜在临床意义。
注：上述文献为示例性内容，实际文献需通过学术数据库(如PubMed)检索确认。
The ELOVL5 antibody is a crucial tool for studying the ELOVL fatty acid elongation family, specifically targeting the ELOVL5 (Elongation of Very Long Chain Fatty Acids Protein 5) enzyme. ELOVL5 catalyzes the elongation of saturated and unsaturated fatty acids, particularly regulating the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFAs) like omega-3 and omega-6 derivatives, which are vital for cell membrane integrity, inflammation signaling, and neuronal function. Dysregulation of ELOVL5 is linked to metabolic disorders, cancer progression, and neurodegenerative diseases, making it a focus in lipid metabolism research.
ELOVL5 antibodies are typically developed in immunized hosts (e.g., rabbits, mice) using synthetic peptides or recombinant protein fragments. These antibodies enable detection of ELOVL5 expression via techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Validated antibodies show specificity through knockout controls or siRNA-mediated knockdown.
Research applications include exploring ELOVL5's role in tumor lipid reprogramming, hepatic steatosis, and retinal function. Commercial antibodies often provide data on species reactivity (human, mouse, rat) and recommended dilutions. Reliable ELOVL5 antibodies are essential for elucidating its tissue-specific functions and potential as a therapeutic target or biomarker in diseases associated with lipid dysregulation.
×
