| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2 |
| Entrez GeneID | 26419 |
| WB Predicted band size | Calculated MW: 44 kDa; Observed MW: 46,54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Recombinant protein of mouse JNK1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3条与JNK1抗体相关的文献示例(均为虚构，仅作格式参考)：
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1. **文献名称**: *JNK1-specific antibody development and its application in stress signaling studies*
**作者**: Davis RJ, Smith JA
**摘要**: 本研究开发了一种高特异性JNK1多克隆抗体，验证了其在Western blot和免疫沉淀中的适用性，并用于检测细胞在氧化应激条件下JNK1的磷酸化激活。
2. **文献名称**: *Differential roles of JNK isoforms in apoptosis revealed by isoform-specific antibodies*
**作者**: Dhanasekaran DN, Minden A
**摘要**: 通过使用JNK1和JNK2亚型特异性抗体，作者证明JNK1在紫外线诱导的细胞凋亡中起主导作用，而JNK2更多参与炎症反应。
3. **文献名称**: *Validation of commercial JNK1 antibodies for human tissue immunohistochemistry*
**作者**: Lee S, et al.
**摘要**: 系统评估了多种市售JNK1抗体在人类肿瘤组织中的特异性，推荐克隆号“SC-571”适用于福尔马林固定石蜡包埋样本的免疫组化检测。
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(注：以上文献为示例，实际引用需查询真实数据库如PubMed)
The c-Jun N-terminal kinase 1 (JNK1), encoded by the *MAPK8* gene, is a member of the mitogen-activated protein kinase (MAPK) family. It plays a critical role in regulating cellular responses to stress signals, including oxidative stress, ultraviolet radiation, cytokines, and metabolic imbalance. JNK1 is activated through phosphorylation by upstream kinases (e.g., MKK4/7) and subsequently phosphorylates transcription factors like c-Jun, ATF2. and p53. modulating processes such as apoptosis, proliferation, differentiation, and inflammation. Dysregulation of JNK1 signaling is implicated in cancer, neurodegenerative disorders, diabetes, and inflammatory diseases.
JNK1 antibodies are essential tools for studying its expression, activation, and localization. These antibodies typically target specific regions of JNK1. such as phosphorylation sites (e.g., Thr183/Tyr185) or distinct epitopes to differentiate JNK1 from its isoforms (JNK2/3). They are widely used in techniques like Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry to assess JNK1 activation status, protein-protein interactions, or tissue distribution. Many commercial JNK1 antibodies are validated for specificity using knockout (KO) controls or isoform-selective assays. Researchers rely on these antibodies to explore JNK1’s role in disease mechanisms or therapeutic targeting, particularly in models of stress-induced pathology. Proper validation remains crucial due to potential cross-reactivity with other MAPKs or splice variants.
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