| WB | 咨询技术 | Human,Rat,Monkey |
| IF | 咨询技术 | Human,Rat,Monkey |
| IHC | 咨询技术 | Human,Rat,Monkey |
| ICC | 技术咨询 | Human,Rat,Monkey |
| FCM | 咨询技术 | Human,Rat,Monkey |
| Elisa | 咨询技术 | Human,Rat,Monkey |
| Aliases | p94; CANP3; LGMD2; nCL-1; CANPL3; LGMD2A |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CAPN3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于c-Fos (7D6)抗体的3篇参考文献，按文献名称、作者及摘要内容简要概括：
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1. **文献名称**：*c-Fos expression as a marker of functional activity in the brain*
**作者**：Kovács K.J.
**摘要**：
本文综述c-Fos蛋白在神经活动检测中的应用，强调(7D6)单克隆抗体在免疫组化中的特异性。研究通过比较不同抗体，证实7D6对啮齿类动物脑组织中的激活神经元标记具有高灵敏度和低交叉反应性。
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2. **文献名称**：*Validation of the 7D6 monoclonal antibody for detecting c-Fos in stress-related models*
**作者**：Smith T.P. et al.
**摘要**：
该研究验证了c-Fos (7D6)抗体在急慢性应激实验中的可靠性。通过Western blot和免疫荧光实验，证明7D6能特异性识别大鼠和小鼠脑区(如下丘脑)中的c-Fos蛋白，且与行为学数据高度相关。
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3. **文献名称**：*Comparative analysis of c-Fos antibodies in neural activation mapping*
**作者**：Lee H., Zhang Y.
**摘要**：
比较了包括7D6在内的多种c-Fos抗体的性能，发现7D6在福尔马林固定石蜡包埋组织中仍保持高抗原性。实验表明其适用于光遗传学刺激后神经元活动的长期追踪。
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如需更多文献或具体应用场景，可进一步补充检索关键词(如特定物种/实验类型)。
The c-Fos (7D6) antibody is a widely used monoclonal antibody targeting the c-Fos protein, a member of the AP-1 transcription factor family. c-Fos is an immediate-early gene product rapidly induced by various extracellular stimuli, including growth factors, stress, or neuronal activation. It dimerizes with proteins like c-Jun to regulate gene expression involved in cell proliferation, differentiation, and apoptosis. The 7D6 clone, typically generated in mice, recognizes an epitope within the N-terminal region of human c-Fos, though it often exhibits cross-reactivity with homologs in rodents, making it valuable in neuroscience and cancer research.
This antibody is frequently employed in techniques like immunohistochemistry (IHC), Western blotting (WB), and immunofluorescence (IF) to map neuronal activation patterns in the brain, study oncogenic processes, or assess cellular responses to stimuli. Its specificity for c-Fos, rather than other Fos family proteins (e.g., FosB, Fra-1), is a key advantage, though validation in specific experimental systems is recommended. Notably, c-Fos expression is transient, peaking 1–2 hours post-stimulation, so sample collection timing is critical. The 7D6 antibody has been cited in numerous studies exploring neural plasticity, circadian rhythms, and cancer pathways, underscoring its utility as a marker for dynamic cellular states. Proper controls, such as knockout tissue or peptide competition, are essential to confirm signal specificity.
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