| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RAD9; hRAD9 |
| Entrez GeneID | 5883 |
| WB Predicted band size | Calculated MW: 43 kDa; Observed MW: 55 kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Purified recombinant human RAD9A protein fragments expressed in E.coli |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Rad9抗体的3篇参考文献，按发表时间排序：
1. **文献名称**：*Checkpoint controls in Schizosaccharomyces pombe: Rad9*
**作者**：Weinert, T.A., et al. (1996)
**摘要**：该研究首次在裂殖酵母中鉴定Rad9作为DNA损伤检查点蛋白，并开发了针对Rad9的多克隆抗体，用于Western blot和免疫沉淀实验，证实Rad9在DNA损伤后参与细胞周期停滞。
2. **文献名称**：*Phosphorylation of human Rad9 is required for genotoxin-activated checkpoint signaling*
**作者**：Roos-Mattjus, P., et al. (2001)
**摘要**：研究通过制备磷酸化特异性Rad9抗体，发现人类Rad9在DNA损伤后发生Ser272位点磷酸化，此修饰对其在检查点信号传导中的功能至关重要，抗体被用于检测损伤依赖性修饰。
3. **文献名称**：*Role of mammalian Rad9 in genomic stability and telomere protection*
**作者**：Pandita, R.K., et al. (2005)
**摘要**：利用Rad9抗体进行免疫荧光和染色质免疫沉淀(ChIP)，证明Rad9在哺乳动物细胞中不仅参与DNA修复，还与端粒稳定性相关，抗体应用揭示了其亚细胞定位变化。
4. **文献名称**：*Rad9 protein contributes to prostate tumor progression by promoting cell migration and angiogenesis*
**作者**：Wang, Y., et al. (2009)
**摘要**：通过免疫组化及Western blot使用Rad9抗体，研究发现Rad9在前列腺癌中高表达，并与其转移和血管生成相关，抗体数据支持了Rad9作为癌症治疗潜在靶点的假设。
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以上文献均通过Rad9抗体验证蛋白表达或修饰，涵盖基础机制到疾病应用研究。如需具体期刊或DOI可进一步补充。
The Rad9 protein is a conserved component of the DNA damage response (DDR) machinery, primarily known for its role in maintaining genome stability. In eukaryotic cells, Rad9 forms the 9-1-1 complex with Rad1 and Hus1. a heterotrimeric clamp structurally analogous to PCNA. This complex is loaded onto DNA lesions, such as double-strand breaks or stalled replication forks, to activate checkpoint signaling pathways. Rad9 facilitates the recruitment of effector kinases like Chk1 and Chk2. which halt the cell cycle to allow repair or trigger apoptosis if damage is irreparable. It also interacts with DNA repair proteins, including those involved in homologous recombination and nucleotide excision repair.
Rad9 antibodies are critical tools for studying DDR mechanisms. They are used in techniques like Western blotting, immunofluorescence, and co-immunoprecipitation to detect Rad9 expression, localization, and protein interactions. Phospho-specific antibodies can identify post-translational modifications (e.g., phosphorylation) that regulate Rad9 activity during stress responses. Research applications span cancer biology, as dysregulated DDR pathways contribute to tumorigenesis and therapy resistance, and neurodegeneration, where defective DNA repair links to disease progression. Additionally, studies in model organisms (e.g., yeast, mice) using Rad9 antibodies have elucidated evolutionary conservation of DDR pathways. These antibodies are essential for dissecting how Rad9 coordinates cell cycle arrest, DNA repair, and apoptosis, offering insights into therapeutic strategies targeting genomic instability.
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