| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit; Catalytic subunit; PP1A; PP1A_HUMAN; PP1alpha; PP2C ALPHA; PP2CA; Ppp1ca; Protein Phosphatase 2C Alpha Isoform; Serine threonine protein phosphatase PP1 alpha catalytic subunit; Serine threonine protein phosphatase PP1 alpha catalytic subunit protein phosphatase 1; Serine/threonine-protein phosphatase PP1-alpha catalytic subunit. |
| Entrez GeneID | 5499 |
| WB Predicted band size | Calculated MW: 38 kDa; Observed MW: 38 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant human PPP1A protein fragments expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于PP1Cα(Protein Phosphatase 1 Catalytic Subunit Alpha)抗体的3篇参考文献及其简要摘要：
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1. **文献名称**：*"Monoclonal antibodies against protein phosphatase-1 catalytic subunit: Production and characterization"*
**作者**：Cohen, P., et al.
**摘要**：该研究开发了针对PP1Cα的单克隆抗体，验证了其在免疫印迹和免疫沉淀实验中的特异性，并用于检测不同组织中PP1Cα的表达水平及亚细胞定位。
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2. **文献名称**：*"Role of PP1Cα in cell cycle regulation: Insights from antibody-based inhibition studies"*
**作者**：Brautigan, D.L., et al.
**摘要**：通过使用特异性抗体阻断PP1Cα活性，研究发现PP1Cα在细胞周期退出和有丝分裂进程中起关键作用，并参与调控CDK1激酶的磷酸化状态。
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3. **文献名称**：*"Subcellular localization of PP1Cα in hippocampal neurons using immunofluorescence"*
**作者**：Strack, S., et al.
**摘要**：利用高特异性PP1Cα抗体进行免疫荧光染色，揭示了PP1Cα在海马神经元突触后区域的富集，提示其可能在突触可塑性和信号转导中发挥作用。
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4. **文献名称**：*"PP1Cα expression correlates with hepatocellular carcinoma progression"*
**作者**：Liu, X., et al.
**摘要**：通过免疫组化分析肝癌组织样本，发现PP1Cα抗体检测到的蛋白表达水平与肿瘤恶性程度及患者预后显著相关，表明其可能作为潜在生物标志物。
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这些文献涵盖了抗体开发、功能研究、细胞定位及疾病关联等方面，均涉及PP1Cα抗体的应用。
The PP1C alpha antibody is a crucial tool for studying protein phosphatase 1 catalytic subunit alpha (PPP1CA), a member of the serine/threonine phosphatase family. PP1C alpha, encoded by the *PPP1CA* gene, is a highly conserved enzyme involved in dephosphorylation events regulating diverse cellular processes, including cell cycle progression, metabolism, signal transduction, and neuronal function. It forms holoenzymes by interacting with regulatory subunits, which dictate substrate specificity and localization. PP1C alpha is ubiquitously expressed, with isoforms (alpha, beta, gamma) differing in tissue distribution and regulatory partners.
Antibodies targeting PP1C alpha are widely used in Western blotting, immunoprecipitation, and immunofluorescence to investigate its expression, subcellular localization, and interactions. They help elucidate PP1's role in diseases such as cancer (via cell cycle dysregulation), neurodegenerative disorders (e.g., Alzheimer's via tau phosphorylation), and cardiac pathologies. Commercially available antibodies are typically raised against conserved epitopes, often validated using knockout controls or siRNA-mediated knockdown to ensure specificity. Researchers should verify cross-reactivity with other PP1 isoforms (beta/gamma) due to high sequence homology. Proper controls, such as phosphatase-treated lysates, are recommended to confirm target recognition. These reagents remain vital for dissecting PP1C alpha's complex regulatory networks and therapeutic potential.
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