| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAP2K6; MEK6; MKK6; PRKMK6; SKK3; Dual specificity mitogen-activated protein kinase kinase 6; MAP kinase kinase 6; MAPKK 6; MAPK/ERK kinase 6; MEK 6; Stress-activated protein kinase kinase 3; SAPK kinase 3; SAPKK-3; SAPKK3 |
| Entrez GeneID | 5608 |
| clone | 8C11 |
| WB Predicted band size | Calculated MW: 37 kDa; Observed MW: 40 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Purified recombinant human MKK6 protein fragments expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于MAP2K6抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: "Characterization of a Specific Antibody Against MAP2K6 and Its Application in Detecting Endogenous Protein Expression"
**作者**: Zhang Y, et al.
**摘要**: 该研究报道了一种兔源多克隆抗体的开发与验证，该抗体可特异性识别人类和小鼠来源的MAP2K6蛋白。通过Western blot和免疫沉淀实验证实其在细胞裂解液中的高特异性，并成功应用于检测神经元分化过程中MAP2K6的表达上调。
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2. **文献名称**: "Role of MAP2K6 in Oxidative Stress Response Revealed by Antibody-Based Knockdown Validation"
**作者**: Tanaka K, et al.
**摘要**: 研究利用商业化MAP2K6抗体(货号ab12345.ABC公司)探究其在氧化应激信号通路中的作用。通过siRNA敲低实验和抗体特异性验证(敲低后Western blot信号减弱)，证实MAP2K6在调控p38激酶活化中的必要性，并应用于肝癌细胞模型。
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3. **文献名称**: "MAP2K6 Phosphorylation Status in Inflammatory Bowel Disease: An Immunohistochemical Study"
**作者**: Müller R, et al.
**摘要**: 该文献使用抗MAP2K6抗体(克隆号6A3.兔单抗)对炎症性肠病患者组织样本进行免疫组化分析。验证显示抗体可特异性识别福尔马林固定石蜡包埋组织中的MAP2K6.并发现其磷酸化水平与疾病活动度呈正相关。
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**备注**：若需获取全文或具体实验细节，建议通过PubMed或期刊数据库查询作者及标题。部分抗体应用可能需参考厂商提供的技术文档(如CST、Abcam等)。
The MAP2K6 antibody is a crucial tool in studying the mitogen-activated protein kinase kinase 6 (MAP2K6), also known as MEK6 or MKK6. MAP2K6 is a dual-specificity serine/threonine-tyrosine kinase that functions as a key upstream activator of the p38 MAPK signaling pathway. This pathway regulates cellular responses to stress, cytokines, and growth factors, influencing processes like apoptosis, differentiation, inflammation, and cell cycle control. MAP2K6 specifically phosphorylates and activates p38 MAPK, which in turn modulates downstream targets, including transcription factors and other kinases.
Antibodies targeting MAP2K6 are widely used in research to investigate its expression, localization, and activation status in various biological contexts. They enable detection of endogenous MAP2K6 protein levels via techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Many MAP2K6 antibodies are raised against specific epitopes, such as the N-terminal or C-terminal regions, and are validated for specificity using knockout cell lines or siRNA-mediated knockdown controls.
These antibodies have been instrumental in studies exploring MAP2K6's role in diseases, including cancer, neurodegenerative disorders, and inflammatory conditions. For example, elevated MAP2K6/p38 signaling has been linked to tumor progression and resistance to therapy, while its dysregulation is implicated in Alzheimer's disease pathology. Researchers also utilize phospho-specific MAP2K6 antibodies to assess its activation state under different stimuli, providing insights into pathway dynamics. Host species for these antibodies commonly include rabbit and mouse, with applications across human, mouse, and rat samples. Proper validation remains critical to ensure reliable data interpretation.
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