| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBG1 |
| Uniprot No | P23258 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-451aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMPREIITLQLGQCGNQIGFEFWKQLCAEHG ISPEGIVEEFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVIHSILNS PYAKLYNPENIYLSEHGGGAGNNWASGFSQGEKIHEDIFDIIDREADGSD SLEGFVLCHSIAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPNQDEMSD VVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQ LVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQ SVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDP TQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLPSAHRVSGLMMAN HTSISSLFERTCRQYDKLRKREAFLEQFRKEDMFKDNFDEMDTSREIVQQ LIDEYHAATRPDYISWGTQEQ |
| 预测分子量 | 53 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TUBG1重组蛋白的假设性参考文献示例(注：内容为模拟生成，非真实文献)：
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1. **标题**: *"Expression and Purification of Recombinant TUBG1 in Escherichia coli for Structural Studies"*
**作者**: Smith A, et al.
**摘要**: 研究报道了在大肠杆菌中高效表达人源TUBG1重组蛋白的优化方法，通过亲和层析纯化获得高纯度蛋白，并利用X射线晶体学解析其三维结构，揭示了其与微管成核相关的关键结构域。
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2. **标题**: *"Functional Analysis of Recombinant TUBG1 in Microtubule Nucleation Using In Vitro Assays"*
**作者**: Chen L, et al.
**摘要**: 通过体外微管组装实验，验证了重组TUBG1蛋白在微管成核中的核心作用，发现其与γ-TuRC复合物其他组分的协同机制，并证明突变体TUBG1可导致微管稳定性下降。
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3. **标题**: *"Recombinant TUBG1 Interaction with Motor Proteins: Implications for Mitotic Spindle Formation"*
**作者**: Tanaka K, et al.
**摘要**: 研究利用重组TUBG1蛋白探究其与驱动蛋白(如Kinesin-5)的相互作用，发现TUBG1在纺锤体组装中的调控功能，并指出其在癌细胞异常分裂中的潜在作用靶点。
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4. **标题**: *"Development of a TUBG1 Recombinant Protein-Based Screening Platform for Anti-Mitotic Drugs"*
**作者**: Wang Y, et al.
**摘要**: 基于昆虫细胞表达系统生产的重组TUBG1蛋白，构建了高通量药物筛选平台，用于鉴定靶向微管成核的小分子抑制剂，为抗癌药物开发提供新策略。
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注：以上文献为示例性质，实际研究中请通过PubMed或专业数据库检索真实文献。
TUBG1 (Tubulin Gamma-1) is a critical member of the γ-tubulin family, primarily recognized for its role in microtubule nucleation and organization. As a core component of the γ-tubulin ring complex (γ-TuRC), it facilitates the assembly of microtubules by acting as a template for α/β-tubulin heterodimers, essential for maintaining cellular structure, intracellular transport, and chromosome segregation during mitosis. TUBG1 is predominantly localized at centrosomes, ensuring proper spindle assembly and cell cycle progression. Dysregulation of TUBG1 has been linked to mitotic errors, genomic instability, and diseases such as cancer or neurodevelopmental disorders.
Recombinant TUBG1 protein is engineered through molecular cloning, typically expressed in *E. coli* or eukaryotic systems like insect or mammalian cells to ensure proper folding and post-translational modifications. This purified protein retains functional properties, enabling *in vitro* studies on microtubule dynamics, interactions with regulatory proteins (e.g., augmin, CDK5RAP2), and mechanisms of γ-TuRC assembly. Researchers leverage it to investigate TUBG1's role in diseases; for instance, overexpression in certain cancers correlates with poor prognosis, while mutations may disrupt neuronal migration.
Its applications extend to drug discovery, particularly screening compounds targeting microtubule disorders. Recombinant TUBG1 also aids in structural studies, such as cryo-EM, to resolve γ-TuRC architecture. Despite advancements, challenges remain in fully recapitulating native complex formation *in vitro*, driving ongoing optimization of expression systems. Overall, recombinant TUBG1 serves as a vital tool for deciphering microtubule biology and developing therapeutic strategies for related pathologies.
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