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产品描述：人α2抗纤溶酶(α2-AP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的SERPINF2含量。SERPINF2是一个靶点。它在人体生理活动中有着重要意义。目前研究主要聚焦于其在细胞信号传导、组织修复等方面的机制。深入探讨它在相关疾病发生发展中扮演的角色，有望为研发针对性的治疗药物和策略提供理论支撑。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础医学研究中纤溶系统功能评估、血栓相关疾病机制探索、肝脏合成功能研究及遗传性纤溶异常疾病的体外分析，同时可为抗凝药物开发提供可靠的生物标志物检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A2AP ELISA Kit; A2AP_HUMAN ELISA Kit; AAP ELISA Kit; Alpha 2 antiplasmin ELISA Kit; Alpha 2 antiplasmin pigment epithelium derived factor ELISA Kit; Alpha 2 AP ELISA Kit; ALPHA 2 PI ELISA Kit; Alpha 2 plasmin inhibitor ELISA Kit; Alpha 2 plasmin inhibitor deficiency ELISA Kit; Alpha-2-antiplasmin ELISA Kit; Alpha-2-AP ELISA Kit; Alpha-2-PI ELISA Kit; Alpha-2-plasmin inhibitor ELISA Kit; Antiplasmin deficiency ELISA Kit; API ELISA Kit; Plasmin inhibitor deficiency ELISA Kit; PLI ELISA Kit; Serine (or cysteine) peptidase inhibitor; clade F; member 2 ELISA Kit; Serine (Or cysteine) peptidase inhibitor; clade F; member 2; isoform CRA_c ELISA Kit; Serine (or cysteine) proteinase inhibitor; clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2 ELISA Kit; Serine (Or cysteine) proteinase inhibitor; clade F; member 2 ELISA Kit; Serine or cysteine peptidase inhibitor clade F member 2 ELISA Kit; Serpin F2 ELISA Kit; Serpin peptidase inhibitor clade F ELISA Kit; Serpin peptidase inhibitor; clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2 ELISA Kit; SERPINF2 ELISA Kit
缩写：SERPINF2
Uniprot No.：P08697
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human α2-AP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-93 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 81-91 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of human α2-AP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 83-94 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.2722.3462.3092.2145001.4761.4911.4841.3892500.8630.8920.8780.7831250.4480.4790.4640.36962.50.2680.2510.2600.16531.20.1870.1780.1830.08815.60.1690.1590.1640.06900.0970.0920.095 | ||||||
货期：3-5 working days
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