| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GRB2; ASH; Growth factor receptor-bound protein 2; Adapter protein GRB2; Protein Ash; SH2/SH3 adapter GRB2 |
| Entrez GeneID | 2885 |
| clone | 6H4 |
| WB Predicted band size | Calculated MW: 25 kDa; Observed MW: 25 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human GRB2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于GRB2抗体的3篇参考文献的简要信息：
1. **文献名称**：*"GRB2: A pivotal adapter protein in signal transduction"*
**作者**：Schlessinger, J.
**摘要**：该综述总结了GRB2在细胞信号转导中的作用，重点讨论了其SH2/SH3结构域介导的蛋白相互作用，并提到使用GRB2抗体阻断其与受体酪氨酸激酶(如EGFR)的结合，以研究下游MAPK通路调控。
2. **文献名称**：*"Monoclonal antibody-based characterization of GRB2 in cancer signaling pathways"*
**作者**：Takahashi, H., et al.
**摘要**：研究开发了针对GRB2不同表位的单克隆抗体，验证了其在免疫沉淀(IP)和免疫印迹(WB)中的应用，并发现GRB2在多种癌症中异常高表达，与肿瘤增殖和转移相关。
3. **文献名称**：*"Targeted disruption of GRB2-EGFR interaction inhibits cell migration"*
**作者**：Sordella, R., et al.
**摘要**：通过GRB2抗体特异性阻断其与EGFR的结合，证明该相互作用对细胞迁移和侵袭至关重要，为靶向GRB2的抗肿瘤策略提供了实验依据。
4. **文献名称**：*"GRB2 antibody screening reveals its role in immune cell activation"*
**作者**：Nishida, M., et al.
**摘要**：利用GRB2抗体进行功能抑制实验，发现GRB2通过调控T细胞受体(TCR)信号通路中的蛋白招募，影响免疫细胞的活化和炎症反应。
这些文献覆盖了GRB2抗体的开发、功能研究及在疾病机制中的应用。
The GRB2 (Growth factor receptor-bound protein 2) antibody is a crucial tool for studying the role of GRB2. an adapter protein involved in intracellular signal transduction. GRB2 contains one SH2 domain and two SH3 domains, enabling it to link activated receptor tyrosine kinases (RTKs) or other phosphorylated signaling molecules to downstream effectors like SOS1. which activates the Ras/MAPK pathway. This protein plays a central role in regulating cell proliferation, differentiation, and survival by mediating signals from growth factor receptors (e.g., EGFR, insulin receptor) to intracellular signaling cascades. Dysregulation of GRB2 is implicated in cancers, immune disorders, and metabolic diseases, making it a focus of oncogenic and therapeutic research.
The GRB2 antibody specifically detects endogenous GRB2 protein in applications such as Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry. It aids in analyzing GRB2 expression levels, post-translational modifications, and interactions with partners like EGFR, Shc, or SOS1. Researchers use this antibody to investigate GRB2's role in pathological contexts, such as tumor metastasis or drug resistance, where aberrant signaling via GRB2-associated pathways often occurs. Validated antibodies ensure specificity for GRB2 isoforms, avoiding cross-reactivity with related adapter proteins. Its utility spans basic research to preclinical studies, supporting mechanistic insights into cellular signaling networks and potential therapeutic targeting.
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