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产品描述：人胶质细胞源性连接蛋白(SERPINE2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SERPINE2含量。人胶质细胞源性连接蛋白（SERPINE2）是一种细胞外丝氨酸蛋白酶抑制剂，主要在胰、结肠和胃的癌症中过表达。它通过调节尿激酶型纤溶酶原激活剂（uPA）和组织型纤溶酶原激活剂（tPA）以及基质金属蛋白酶活性，在止血、细胞粘附和肿瘤转移中发挥作用。SERPINE2在生理和病理过程中扮演重要角色，包括神经系统功能、止血、血管生成和生殖过程。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础科研中探索SERPINE2在组织纤维化模型中的表达动态、肿瘤微环境相关信号通路研究，或神经系统疾病中胶质细胞功能异常的分子机制分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GDN ELISA Kit; GDN_HUMAN ELISA Kit; GDNPF ELISA Kit; Glia derived nexin ELISA Kit; Glia-derived nexin ELISA Kit; Glial derived neurite promoting factor ELISA Kit; P17 ELISA Kit; Peptidase inhibitor 7 ELISA Kit; Pi-7 ELISA Kit; Plasminogen activator inhibitor type 1; member 2 ELISA Kit; PN-1 ELISA Kit; PN1 ELISA Kit; PNI ELISA Kit; Protease inhibitor 7 ELISA Kit; Protease nexin 1 ELISA Kit; Protease nexin I ELISA Kit; Serpin E2 ELISA Kit; Serpin family E member 2 ELISA Kit; Serpin peptidase inhibitor; clade E (nexin; plasminogen activator inhibitor type 1); member 2 ELISA Kit; SERPINE 2 ELISA Kit; Serpine2 ELISA Kit
缩写：SERPINE2
Uniprot No.：P07093
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 85 | |||||
| Range % | 82-87 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 93-99 | ||||||
| 1:4 | Average % | 98 | |||||
| Range % | 94-103 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 93-100 | ||||||
回收率：
| The recovery of human SERPINE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 88 | 84-92 | |||||
| EDTA plasma (n=4) | 107 | 103-110 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.2812.1082.1952.065101.4051.4321.4191.28950.8220.8010.8120.6822.50.4830.4680.4760.3461.250.3650.3720.3690.2390.6250.2870.2980.2930.1630.3120.2220.2200.2210.09100.1310.1290.130 | |||||||
货期：3-5 working days
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