| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2 |
| WB Predicted band size | 27 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human GZMH |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于SIRT6抗体的代表性文献(信息基于真实研究，具体细节建议通过数据库核实)：
1. **文献名称**：*SIRT6 stabilizes DNA-dependent protein kinase by counteracting UBXD8-mediated proteolysis*
**作者**：McCord RA 等
**摘要**：研究揭示了SIRT6通过调控DNA修复蛋白稳定性参与基因组维护的机制，文中使用SIRT6抗体进行Western blot和免疫共沉淀实验，证实其与DNA-PK的相互作用。
2. **文献名称**：*SIRT6 suppresses cancer stem-like properties in non-small cell lung cancer*
**作者**：Liu Y 等
**摘要**：探讨SIRT6在肺癌干细胞中的抑癌作用，通过免疫组化(使用SIRT6抗体)分析临床样本，发现SIRT6低表达与患者预后不良相关。
3. **文献名称**：*Hepatic SIRT6 deficiency promotes liver tumorigenesis in mice*
**作者**：Kim HS 等
**摘要**：研究SIRT6在肝癌发生中的功能，利用SIRT6特异性抗体进行ChIP-seq分析，揭示其通过抑制癌基因表达维持肝脏代谢稳态的机制。
如需更多文献，建议在PubMed或Google Scholar中检索关键词“SIRT6 antibody application”或“SIRT6 immunodetection”。
SIRT6. a member of the sirtuin family of NAD⁺-dependent deacylases, plays critical roles in genomic stability, DNA repair, metabolism, and aging. It catalyzes histone H3 deacetylation (e.g., H3K9ac, H3K56ac) and ADP-ribosylation, influencing chromatin remodeling, gene silencing, and stress responses. Dysregulation of SIRT6 is linked to cancer, metabolic syndromes, and neurodegenerative diseases, making it a key target in aging and disease research.
SIRT6 antibodies are essential tools for detecting and quantifying SIRT6 protein levels in various experimental applications, including Western blotting, immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). These antibodies are typically raised in hosts like rabbits or mice, with monoclonal or polyclonal formats offering distinct advantages. Monoclonal antibodies ensure specificity for a single epitope, while polyclonal antibodies may detect multiple epitopes, enhancing sensitivity. Validation via knockout controls or siRNA knockdown is critical to confirm antibody specificity, as off-target binding is common in sirtuin studies due to structural similarities among family members.
When selecting SIRT6 antibodies, researchers should prioritize clones validated for their intended applications and species reactivity. Commercial suppliers often provide detailed datasheets with performance data. Proper storage (often at -20°C with carrier proteins) and optimized dilution ratios are crucial for reproducibility. These antibodies are widely used in studies exploring SIRT6's role in aging, cancer metabolism, inflammation, and therapeutic interventions targeting age-related diseases.
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