| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TTC33 |
| Uniprot No | Q6PID6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-262aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMASFGWKRKIGEKVSKVTSQQFEAEAA DEKDVVDNDEGNWLHAIKRRKEILLEGCAEKSKQLKDEGASLAENKRYRE AIQKWDEALQLTPNDATLYEMKSQVLMSLHEMFPAVHAAEMAVQQNPHSW ESWQTLGRAQLGLGEIILAIRSFQVALHIYPMNPEIWKEDLSWARTLQEQ QKVAQRIKKSEAPAEVTHFSPKSIPDYDFESDEIVAVCAAIAEKEKTVSA NKTMVIVSASGAIETVTEKEDGATPPDGSVFIKAR |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是生成的示例参考文献(注：以下内容为模拟示例，实际文献请通过学术数据库查询)：
1. **"Functional characterization of recombinant TTC33 protein in cellular protein synthesis regulation"**
- Authors: Zhang L, et al.
- 摘要：研究通过大肠杆菌系统重组表达TTC33蛋白，发现其通过与核糖体亚基相互作用调控翻译延伸，并影响细胞应激条件下的蛋白质质量控制。
2. **"Structural analysis and disease-associated mutations of human TTC33"**
- Authors: Gupta R, et al.
- 摘要：解析了重组TTC33蛋白的晶体结构，结合生信分析揭示其在遗传性疾病中的突变热点，为功能缺失机制提供分子基础。
3. **"TTC33 knockdown and recombinant rescue in zebrafish neurodevelopmental models"**
- Authors: Müller S, et al.
- 摘要：利用重组TTC33蛋白在斑马鱼模型中恢复基因敲除导致的神经管缺陷，证实其在胚胎发育中的保守功能。
4. **"High-yield production of recombinant TTC33 for drug screening applications"**
- Authors: Kim J, et al.
- 摘要：优化哺乳动物细胞表达系统获得高纯度TTC33.用于筛选靶向该蛋白的小分子抑制剂，推进癌症治疗研究。
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建议通过 **PubMed、Web of Science 或 Google Scholar** 检索真实文献，关键词可尝试：
`TTC33 recombinant protein` `TTC33 gene function` `TTC33 structure`
如需文献获取协助，请提供更多具体方向(如疾病关联或分子机制)。
**Background of TTC33 Recombinant Protein**
TTC33 (Tetratricopeptide Repeat Domain 33) is a protein encoded by the *TTC33* gene, characterized by its conserved tetratricopeptide repeat (TPR) motifs. These structural domains facilitate protein-protein interactions, enabling TTC33 to participate in diverse cellular processes, including intracellular trafficking, signal transduction, and chaperone-mediated protein folding. While its exact physiological role remains under investigation, TTC33 has been implicated in regulating the Wnt/β-catenin signaling pathway, a critical pathway in development, tissue homeostasis, and cancer. Dysregulation of TTC33 expression has been observed in certain cancers, suggesting a potential role as a tumor suppressor or oncogenic modulator, though its mechanistic contributions are context-dependent.
Recombinant TTC33 protein is engineered for *in vitro* and *in vivo* studies to elucidate its molecular functions. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains functional TPR domains, enabling researchers to study its interaction partners, structural dynamics, and regulatory mechanisms. Recent studies highlight its involvement in cellular stress responses, such as endoplasmic reticulum (ER) stress and autophagy, linking it to diseases like hepatocellular carcinoma and colorectal cancer.
Despite progress, challenges persist in defining TTC33's precise roles due to conflicting findings across studies and limited tissue-specific data. Recombinant TTC33 serves as a vital tool to address these gaps, offering insights into its potential as a therapeutic target or diagnostic biomarker. Further research is needed to unravel its interplay with signaling networks and its implications in both physiological and pathological contexts.
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