| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSTD1 |
| Uniprot No | Q8NFU3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-115aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMAGAPTVSLPELRSLLASGRARLFDVRSRE EAAAGTIPGALNIPVSELESALQMEPAAFQALYSAEKPKLEDEHLVFFCQ MGKRGLQATQLARSLGYTGARNYAGAYREWLEKES |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的关于TSTD1重组蛋白的参考文献示例(实际文献需通过学术数据库核实)：
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1. **文献名称**: *"Recombinant Expression and Functional Characterization of Human TSTD1 in Antioxidant Defense"*
**作者**: Zhang L, et al. (2022)
**摘要**: 本研究成功在大肠杆菌中表达并纯化人源TSTD1重组蛋白，证实其通过硫代硫酸盐代谢途径增强细胞对氧化应激的抵抗能力，为抗氧化治疗提供潜在靶点。
2. **文献名称**: *"Structural Insights into the Catalytic Mechanism of TSTD1 through Crystallographic Analysis"*
**作者**: Watanabe K, et al. (2020)
**摘要**: 通过X射线晶体学解析TSTD1重组蛋白的3D结构，揭示其活性位点关键氨基酸残基在硫转移反应中的作用机制，为酶活性改造提供依据。
3. **文献名称**: *"TSTD1 Knockdown Exacerbates Cisplatin-Induced Nephrotoxicity: Implications for Drug-Induced Renal Injury"*
**作者**: Gupta S, et al. (2021)
**摘要**: 利用重组TSTD1蛋白进行体外实验，证明其通过调节硫代谢通路减轻顺铂诱导的肾小管细胞损伤，提示其在化疗副作用中的保护作用。
4. **文献名称**: *"Development of a High-Yield TSTD1 Recombinant Protein Production System in Pichia pastoris"*
**作者**: Chen H, et al. (2019)
**摘要**: 优化毕赤酵母表达系统实现TSTD1重组蛋白的高效分泌表达，纯度达95%以上，为工业化生产及药物筛选奠定基础。
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**备注**：以上文献信息为模拟生成，实际研究需通过PubMed、Web of Science或Google Scholar等平台检索关键词“TSTD1 recombinant protein”或“thiosulfate sulfurtransferase”获取真实数据。
Thiosulfate sulfurtransferase (TSTD1), also known as rhodanese, is a mitochondrial enzyme involved in sulfur metabolism and detoxification pathways. It catalyzes the transfer of sulfur atoms from thiosulfate (S₂O₃²⁻) to cyanide or other acceptors, playing a critical role in cellular defense against toxic sulfides and cyanide exposure. TSTD1 is particularly notable for its contribution to maintaining redox homeostasis, especially under hypoxic or mitochondrial stress conditions. Its enzymatic activity generates hydrogen sulfide (H₂S), a gasotransmitter with signaling functions in vasodilation, anti-inflammatory responses, and cytoprotection.
Recombinant TSTD1 protein is produced using biotechnological platforms, such as *E. coli* or mammalian expression systems, to enable functional and structural studies. Purification typically involves affinity chromatography tags (e.g., His-tag) for high yield and purity. Research on recombinant TSTD1 has expanded due to its potential therapeutic relevance. For instance, H₂S derived from TSTD1 activity has been implicated in mitigating ischemia-reperfusion injury, modulating apoptosis, and enhancing cellular adaptation to oxidative stress. Dysregulation of TSTD1 expression or activity is linked to pathologies like cardiovascular diseases, neurodegenerative disorders, and cancer, making it a target for drug development.
Despite progress, the precise mechanisms of TSTD1 in disease contexts remain unclear. Studies using recombinant proteins aim to elucidate its interaction networks, substrate specificity, and regulatory roles in sulfur-dependent pathways. Additionally, engineered variants of TSTD1 are explored for biocatalytic applications or as biosensors for sulfide detection. Ongoing research continues to uncover its multifaceted roles in cellular physiology, highlighting TSTD1 as a promising candidate for both biomedical and industrial innovations.
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