| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSSK6 |
| Uniprot No | Q9BXA6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-273aa |
| 氨基酸序列 | MSGDKLLSELGYKLGRTIGEGSYSKVKVATSKKYKGTVAIKVVDRRRAPP DFVNKFLPRELSILRGVRHPHIVHVFEFIEVCNGKLYIVMEAAATDLLQA VQRNGRIPGVQARDLFAQIAGAVRYLHDHHLVHRDLKCENVLLSPDERRV KLTDFGFGRQAHGYPDLSTTYCGSAAYASPEVLLGIPYDPKKYDVWSMGV VLYVMVTGCMPFDDSDIAGLPRRQKRGVLYPEGLELSERCKALIAELLQF SPSARPSAGQVARNCWLRAGDSG |
| 预测分子量 | 57 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TSSK6重组蛋白的模拟参考文献示例(实际文献需通过学术数据库查询)：
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1. **文献名称**：*Expression and purification of recombinant human TSSK6 kinase in Escherichia coli*
**作者**：E. Shang et al.
**摘要**：研究成功构建了TSSK6的重组表达质粒，通过大肠杆菌系统高效表达可溶性蛋白，并利用亲和层析纯化。通过体外激酶实验验证其磷酸化活性，为后续功能研究奠定基础。
2. **文献名称**：*Functional characterization of TSSK6 in spermatogenesis using recombinant protein assays*
**作者**：J. Xu et al.
**摘要**：利用重组TSSK6蛋白进行体外磷酸化实验，发现其特异性磷酸化精子发生相关底物(如SPAG6)，并揭示TSSK6缺失导致小鼠精子鞭毛结构异常，提示其在精子成熟中的关键作用。
3. **文献名称**：*TSSK6 knockout and recombinant protein rescue in male infertility models*
**作者**：R. Li et al.
**摘要**：通过构建TSSK6基因敲除小鼠模型，发现雄性不育表型;体外补充重组TSSK6蛋白可部分恢复精子活力，表明其在睾丸微环境中的潜在治疗价值。
4. **文献名称**：*Structural insights into TSSK6 catalytic domain by X-ray crystallography*
**作者**：K. Tanaka et al.
**摘要**：解析了重组TSSK6激酶结构域的晶体结构，揭示其ATP结合口袋特征，为开发靶向TSSK6的男性避孕药物提供结构生物学依据。
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**注意**：以上内容为模拟生成，实际文献请通过PubMed、Web of Science或Google Scholar检索关键词“TSSK6 recombinant protein”获取。
TSSK6 (Testis-specific serine kinase 6) is a member of the serine/threonine kinase family, primarily expressed in the testis and implicated in spermatogenesis. Discovered for its testis-specific expression pattern, TSSK6 is localized to post-meiotic germ cells, particularly during the later stages of sperm development, such as spermatid elongation and maturation. Its expression is tightly regulated, peaking during spermiogenesis, where it plays a critical role in chromatin condensation, acrosome formation, and sperm motility. Structurally, TSSK6 contains a conserved kinase domain and a short N-terminal region, with phosphorylation activity essential for its biological functions.
Recombinant TSSK6 protein is produced using genetic engineering techniques, often expressed in bacterial or mammalian systems to study its enzymatic activity, substrate interactions, and regulatory mechanisms. Purification methods typically involve affinity chromatography to ensure high purity and functionality. Researchers utilize recombinant TSSK6 to investigate its role in male fertility, as knockout studies in mice have demonstrated that TSSK6 deficiency leads to impaired sperm maturation, reduced motility, and infertility. These findings highlight its potential as a biomarker for male infertility or a therapeutic target for contraceptive development.
Current research explores TSSK6's interaction with proteins like TSKS (TSSK6-binding protein) and its phosphorylation of substrates involved in chromatin remodeling. Its testis-specificity and essential role in spermatogenesis make it a promising candidate for non-hormonal male contraception. However, further studies are needed to elucidate its full signaling network and clinical applicability. Overall, TSSK6 recombinant protein serves as a vital tool for advancing reproductive biology and translational medicine.
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